FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656449
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG40040.6099483737502838No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34830.530581964478581No Hit
ACCTATATGGATCTGAATAGCCTGAGATCTGAGGACACGGCCGTCTATTT29050.44253247396218137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22850.3480849235812683No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20810.3170086328107743No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA19660.2994901355627017No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19610.2987284617693073No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC18100.2757259132087946No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17040.259578428788832No Hit
CATATAGGTTGTACTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT16950.258207415960722No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16580.252571029889603No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16440.2504383432680985No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15900.24221226629943834No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15690.23901323636718164No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15470.23566187167624597No Hit
ACTTACTACTGTCTGCAGAGTTACAGTTCCACTCGTTCTTTTGGCCAGGG15430.23505253264153042No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14990.22834980325965917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14570.2219517433951457No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14380.21905738298024674No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14130.21524901401327443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13670.2082416151140454No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13440.2047379156644309No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA13130.20001553814538525No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12950.1972735124891652No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12730.19392214779822958No Hit
GAAATAGACGGCCGTGTCCTCAGATCTCAGGCTATTCAGATCCATATAGG12700.1934651435221929No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12130.18478206227749605No Hit
TCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGC12050.1835633842080649No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11960.1821923713799549No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11550.17594664627412032No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11490.17503263772204694No Hit
ATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT11470.17472796820468917No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11380.17335695537657914No Hit
TTACTACTGTCTGCAGAGTTACAGTTCCACTCGTTCTTTTGGCCAGGGGA11310.17229061206582688No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGAAGCACAGCTCAT11090.16893924737489127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10930.16650189123602901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10670.1625411875103778No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10540.16056083564755222No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10360.15781880999133213No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10320.15720947095661658No Hit
GTCTATTTCTGTGCAATCGTGACTACGGTGACCACTGTCGTTGACTACTG10250.15614312764586433No Hit
CCTCTAAAGAAGCCCCTGGAAGCACAGCTCATCACCATGGACTGGACCTG10110.15401044102435985No Hit
GTCTTTGGTAGAGCAACCTACGCACAAAAGTTCCAGGGCAGAGTCACGAT9950.1515730848854976No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9930.15126841536813979No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTGTCTTTGGT9790.1491357287466353No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9770.14883105922927753No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9550.14547969453834192No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGTTTGGTCCCCTGGCCAAAAGAA9430.14365167743419519No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9400.1431946731581585No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9340.14228066460608518No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG9300.14167132557136958No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9240.14075731701929625No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8890.135425600465535No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8650.13176956625724162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8590.13085555770516827No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8550.1302462186704527No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8500.12948454487705824No Hit
CCTGTAAGGCTTCTGGAGGCACCTTCAGCAGCAATGTTTTCAATTGGGTG8500.12948454487705824No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8480.12917987535970044No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8460.12887520584234266No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8440.12857053632498489No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT8430.12841820156630598No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8410.1281135320489482No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8370.12750419301423263No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCTGC8220.12521917163404925No Hit
ATTCAGATCCATATAGGTTGTACTCGTGGATTCGTCCGCGGTAATCGTGA8120.12369582404726034No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG8110.12354348928858144No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8040.1224771459778292No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8020.12217247646047141No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7940.12095379839104027No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7940.12095379839104027No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7920.12064912887368248No Hit
CAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGT7900.1203444593563247No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7860.11973512032160914No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7800.11882111176953578No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7640.1163837556306735No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCTGCAGAGTTACAGT7610.11592675135463684No Hit
ATATGGATCTGAATAGCCTGAGATCTGAGGACACGGCCGTCTATTTCTGT7600.11577441659595794No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG7510.11440340376784792No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAAGTTTGGTCC7470.11379406473313236No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7450.11348939521577457No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC7410.112880056181059No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGG7380.11242305190502233No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7310.11135670859427009No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7280.1108997043182334No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA7240.11029036528351784No Hit
CTCCTGATCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT7180.10937635673144447No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7140.10876701769672892No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT7130.10861468293805003No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC7080.10785300914465556No Hit
GATCTGAATAGCCTGAGATCTGAGGACACGGCCGTCTATTTCTGTGCAAT7060.10754833962729779No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7000.10663433107522442No Hit
GTTACAGTTCCACTCGTTCTTTTGGCCAGGGGACCAAACTTGAGATCAAA6960.10602499204050886No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG6960.10602499204050886No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6950.10587265728182996No Hit
GTCGTTGACTACTGGGGCCAGGGAACCCTGGTCACTGTCTCCTCAGCCTC6890.10495864872975662No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6860.10450164445371993No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG6850.10434930969504105No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA6800.10358763590164659No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA6800.10358763590164659No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6770.10313063162560991No Hit
ACCAAAGACAGGGATGATTCCTCCCATCCACTCAAGCCCTTGTCCAGGGG6760.10297829686693102No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6750.10282596210825212No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6700.10206428831485766No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG6680.10175961879749988No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6660.10145494928014209No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC6630.10099794500410542No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA6610.10069327548674764No Hit
GAGTTACAGTTCCACTCGTTCTTTTGGCCAGGGGACCAAACTTGAGATCA6600.10054094072806875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.085.3237841
TATCAAC15850.073.629762
TCAACGC16100.072.409084
ATCAACG16050.072.233093
CAACGCA16600.070.228085
AACGCAG17350.067.583896
CGCAGAG19800.059.221258
GTGCTAG2350.051.8173331
ACGCAGA23800.049.268097
GAATACG2200.048.72329
CTAGAGA2450.043.7881364
GCTAGAG2550.042.0966263
CAGCGCT4950.041.8658649
GCAGCGC5300.040.471038
CATGGGG9150.037.5190164
ACATGGG18150.037.4578863
GTACATG18150.037.097551
TACATGG18250.036.877382
GGCAGCG5850.036.6660587
ATGGGGC1854.771209E-934.7938165
Produced by FastQC (version 0.11.5)