FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656449
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG37060.5645526156639739No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32980.502400034122986No Hit
ACCTATATGGATCTGAATAGCCTGAGATCTGAGGACACGGCCGTCTATTT22830.34778025406391055No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20450.31152458149833423No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT19800.3016228221842062No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18450.2810576297625558No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA18140.27633525224351013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17400.26506248010127215No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16010.24388794864490618No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15750.23992724491925496No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15590.2374898887803927No Hit
CATATAGGTTGTACTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT15060.2294161465704114No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC14930.22743579470758582No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14270.21738170063477893No Hit
ACTTACTACTGTCTGCAGAGTTACAGTTCCACTCGTTCTTTTGGCCAGGG13700.2086986193900821No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13600.20717527180329318No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13600.20717527180329318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13490.20549958945782534No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA13390.2039762418710364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13200.2010818814561375No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13160.2004725424214219No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12830.19544549538501851No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12170.1853914013122116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11990.18264937565599157No Hit
GAAATAGACGGCCGTGTCCTCAGATCTCAGGCTATTCAGATCCATATAGG11750.17899334144769816No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11080.16878691261621237No Hit
ATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT10900.1660448869599923No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10830.16497854364924008No Hit
TCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGC10800.1645215393732034No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10760.16391220033848783No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10700.1629981917864145No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10520.16025616613019442No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10380.15812347950868993No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10360.15781880999133213No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10240.15599079288718545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10200.15538145385246988No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10170.1549244495764332No Hit
TTACTACTGTCTGCAGAGTTACAGTTCCACTCGTTCTTTTGGCCAGGGGA10100.15385810626568097No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9630.14669837260777302No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9530.14517502502098412No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGAAGCACAGCTCAT9290.1415189908126907No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9210.14030031274325955No Hit
GTCTTTGGTAGAGCAACCTACGCACAAAAGTTCCAGGGCAGAGTCACGAT9120.13892929991514955No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9030.13755828708703952No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8960.13649194377628726No Hit
CCTCTAAAGAAGCCCCTGGAAGCACAGCTCATCACCATGGACTGGACCTG8860.13496859618949833No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGTTTGGTCCCCTGGCCAAAAGAA8790.1339022528787461No Hit
GTCTATTTCTGTGCAATCGTGACTACGGTGACCACTGTCGTTGACTACTG8700.13253124005063607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8680.13222657053327827No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8630.13146489673988382No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8320.12674251922083818No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTGTCTTTGGT8150.12415282832329701No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC8110.12354348928858144No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8040.1224771459778292No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8030.12232481121915031No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7970.12141080266707695No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7960.12125846790839807No Hit
ATTCAGATCCATATAGGTTGTACTCGTGGATTCGTCCGCGGTAATCGTGA7850.11958278556293024No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7840.11943045080425137No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCTGC7690.11714542942406797No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7570.11531741231992126No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7530.1147080732852057No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7500.11425106900916904No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7480.11394639949181123No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7300.11120437383559119No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG7270.1107473695595545No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7220.10998569576616006No Hit
CAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGT7210.10983336100748116No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCTGCAGAGTTACAGT7170.1092240219727656No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7160.1090716872140867No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA7150.10891935245540782No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7120.10846234817937113No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7060.10754833962729779No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC7050.10739600486861889No Hit
CCTGTAAGGCTTCTGGAGGCACCTTCAGCAGCAATGTTTTCAATTGGGTG7040.10724367010993999No Hit
GTCCAGGGGCCTGTCGCACCCAATTGAAAACATTGCTGCTGAAGGTGCCT6930.10556798776447218No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA6870.10465397921239883No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6870.10465397921239883No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG6840.10419697493636215No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAAGTTTGGTCC6800.10358763590164659No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG6770.10313063162560991No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6770.10313063162560991No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6750.10282596210825212No Hit
ACCAAAGACAGGGATGATTCCTCCCATCCACTCAAGCCCTTGTCCAGGGG6730.10252129259089435No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT6720.10236895783221545No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGG6700.10206428831485766No Hit
CTCCTGATCTATACTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT6670.101607284038821No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6620.10084561024542654No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)