FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671752
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCATCTTCAGGGAGA42580.6338648787052364No Hit
GTCTATTATAGTGGTAACACCTACTATAGTGACAGCTCCAACTACACCCC30510.45418547321035146No Hit
GTCTATTACTGTGTGAGACTTCAGTATTGTAGCAGTAGCAGTTGCTTTGA27940.4159273065059725No Hit
ATAATAGACATTGCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA15860.23609903654920267No Hit
CTATTATAGTGGTAACACCTACTATAGTGACAGCTCCAACTACACCCCGT15590.2320796960783146No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14090.20975002679560315No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13320.1982874632304779No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT12260.18250783027069514No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12120.18042372780430874No Hit
TCCATAGACACGTCCATGAATCAGTTCTCCCTGAAGATGACCTCTGTGAC11560.1720873179387631No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCATCTTCAGGGA11270.16777024854410558No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGGTTCCTACTGGGGCTGGATCCG10750.1600292965260989No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10640.15839178744536675No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10630.158242922983482No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10590.15764746513594302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.15645654944086507No Hit
GTTCTGGGATTCATCAAAGCAACTGCTACTGCTACAATACTGAAGTCTCA9410.14008145863354332No Hit
CACCTACTATAGTGACAGCTCCAACTACACCCCGTCCCTCAGGAGTCGAG9390.13978372970977385No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG9280.13814622062904167No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9190.136806440472079No Hit
CTATTACTGTGTGAGACTTCAGTATTGTAGCAGTAGCAGTTGCTTTGATG8870.1320427776917672No Hit
GTCCCACACCTGGCAATAATAGTCACCCTCATCCCCGACTTGGGGTCTGC8680.12921435291595706No Hit
GTCTATGGATATGGTGACTCGACTCCTGAGGGACGGGGTGTAGTTGGAGC8620.1283211661446486No Hit
CTACTATAGTGACAGCTCCAACTACACCCCGTCCCTCAGGAGTCGAGTCA8380.12474841905941478No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT8150.12132453643606568No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGTGTTGCTG8120.12087794305041147No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7770.11566768688444544No Hit
GTACTGGTTCCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTG7580.11283926210863533No Hit
CTATAATAGACATTGCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG7300.1086710571758625No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT7250.1079267348664388No Hit
TCCCAGAACTGGTTCGACCCGTGGGGCCAGGGAACCCTGGTCGCCGTCTC7250.1079267348664388No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA7180.1068846836332456No Hit
ACTACACCCCGTCCCTCAGGAGTCGAGTCACCATATCCATAGACACGTCC6960.10360966547178126No Hit
CCTATGAGTTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG6860.10212102085293381No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA6820.10152556300539485No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT6820.10152556300539485No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG6810.10137669854351011No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGTTGACTCAGCCACTCTCAGTGT6720.10003691838654741No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC502.080245E-457.596934
GTATCAA17650.053.8642041
GGTATCA9600.050.2660331
ATCAACG19450.047.0173533
TCAACGC19600.046.6505744
CAACGCA19850.046.0630385
TATCAAC20650.044.6338122
AACGCAG20550.044.493986
ATACCGC657.593947E-444.3020327
CGCAGAG20950.043.6412058
GAATACG1054.0119594E-641.1376049
GCAGAGT23950.038.4752549
CAGCCGT16100.037.113279
ACAGCCG16600.036.4290858
GTAATAG18350.034.1471331
ACGCAGA27350.033.4290057
TAGACAG18650.033.1993035
GACAGCC18900.033.1386267
GTGCTAG1601.9750732E-631.5100521
AGTACGG950.004890179330.3141775
Produced by FastQC (version 0.11.5)