FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671752
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCATCTTCAGGGAGA41360.6157034143552979No Hit
GTCTATTATAGTGGTAACACCTACTATAGTGACAGCTCCAACTACACCCC30470.45359001536281246No Hit
GTCTATTACTGTGTGAGACTTCAGTATTGTAGCAGTAGCAGTTGCTTTGA29210.4348330931653348No Hit
CTATTATAGTGGTAACACCTACTATAGTGACAGCTCCAACTACACCCCGT15820.23550357870166372No Hit
ATAATAGACATTGCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA14970.22285009944146053No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14280.21257845157141328No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT12690.18890900213173908No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12640.18816467982231538No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11780.17536233610022747No Hit
TCCATAGACACGTCCATGAATCAGTTCTCCCTGAAGATGACCTCTGTGAC11450.17044980885803093No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGGTTCCTACTGGGGCTGGATCCG10940.16285772130190904No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10900.16226226345437006No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCATCTTCAGGGA10750.1600292965260989No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10690.15913610975479045No Hit
GTTCTGGGATTCATCAAAGCAACTGCTACTGCTACAATACTGAAGTCTCA9740.14499398587573986No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9440.14052805201919755No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9310.1385928140146959No Hit
CTATTACTGTGTGAGACTTCAGTATTGTAGCAGTAGCAGTTGCTTTGATG9090.13531779585323156No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG8980.13368028677249938No Hit
GTCTATGGATATGGTGACTCGACTCCTGAGGGACGGGGTGTAGTTGGAGC8770.13055413307291977No Hit
GTCCCACACCTGGCAATAATAGTCACCCTCATCCCCGACTTGGGGTCTGC8480.1262370636782622No Hit
CTACTATAGTGACAGCTCCAACTACACCCCGTCCCTCAGGAGTCGAGTCA8260.12296204551679787No Hit
CACCTACTATAGTGACAGCTCCAACTACACCCCGTCCCTCAGGAGTCGAG8250.12281318105491312No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA7950.11834724719837082No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACAGTGTTGCTG7890.11745406042706237No Hit
TCCCAGAACTGGTTCGACCCGTGGGGCCAGGGAACCCTGGTCGCCGTCTC7590.11298812657052008No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7550.11239266872298109No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT7510.11179721087544213No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7260.10807559932832354No Hit
CTATAATAGACATTGCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG7180.1068846836332456No Hit
CCTATGAGTTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG7160.10658695470947611No Hit
ACTACACCCCGTCCCTCAGGAGTCGAGTCACCATATCCATAGACACGTCC6990.1040562588574355No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT6970.103758529933666No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG6920.10301420762424227No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT6850.10197215639104906No Hit
GTGTGGGACAGCAACACTGTGATTTTCGGCGGAGGGACCAAGCTGACCGT6830.1016744274672796No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGG6800.10122783408162536No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCAC6780.10093010515785587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)