FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838964
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27180.3239709927958768No Hit
GACCTAGGACGGTCAGAATGGTCCCTCCGCCGAACACCCAAGTGTAGGTG18960.22599301042714584No Hit
GTGATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG17280.2059683133006899No Hit
GTATATCACTGTGCGAGAGTAAAGATAACAGCAGCTGGTCCTAACTACTA16060.1914265689588588No Hit
GCCTTGGGCTGACCTAGGACGGTCAGAATGGTCCCTCCGCCGAACACCCA14040.16734925455681054No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13900.16568052979627254No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13890.16556133517051985No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13680.16305824802971283No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAACAGTGATATT13670.16293905340396012No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAA13280.15829046299960428No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13070.1557873758587973No Hit
GTTCATATGCAGGCACCTACACTTGGGTGTTCGGCGGAGGGACCATTCTG12920.15399945647250657No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12550.14958925531965614No Hit
GATTATTACTGCTGTTCATATGCAGGCACCTACACTTGGGTGTTCGGCGG12470.14863569831363443No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12020.1432719401547623No Hit
CTCCTGCACTGGAACCAACAGTGATATTGGTGGTTATACCTATGTCTCCT12010.14315274552900958No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11920.14207999389723516No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGCTCTCCTGCA11670.1391001282534173No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGTTCATAT11510.1371930142413739No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC11290.1345707324748142No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11290.1345707324748142No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11280.13445153784906147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11160.1330212023400289No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCGGTCTGCCCTGACTCAGCCT11140.13278281308852347No Hit
GGTTATACCTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA11130.13266361846277075No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10800.1287301958129312No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10640.12682308180088775No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10550.12575033016911333No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10510.1252735516661025No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10460.1246775785373389No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10300.12277046452529548No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10270.12241288064803735No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10200.12157851826776833No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9840.11728751174067065No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG9800.11681073323765978No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9690.11549959235437991No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9610.11454603534835821No Hit
ACCTAGGACGGTCAGAATGGTCCCTCCGCCGAACACCCAAGTGTAGGTGC9540.11371167296808922No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9430.11240053208480935No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9400.11204294820755122No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9220.10989744494400236No Hit
GCCGTATATCACTGTGCGAGAGTAAAGATAACAGCAGCTGGTCCTAACTA9150.10906308256373337No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCGGTCTGCCCTG9120.10870549868647524No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9050.10787113630620622No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8990.10715596855168995No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8900.10608321691991551No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8570.10214979427007596No Hit
GTGTAGGTGCCTGCATATGAACAGCAGTAATAATCAGCCTCATCCTCAGC8470.10095784801254881No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT8440.10060026413529068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)