FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193595
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAATTGGCCGCCCCTTTTCGGCCCTGGGACCAAAGTGGATATCA32350.2710299557219995No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT30260.25351982875263385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28190.23617726280689846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17360.14544296851109464No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC16900.1415890649675979No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16820.1409188208730767No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCT16470.13798650295954656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15260.12784906102991384No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAACTTAGCCTGGTACCAAC12610.10564722539889997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14200.059.8312454
ATCAACG15750.053.9430923
CAACGCA15850.053.6027575
GTATCAA17950.050.540611
AACGCAG17300.049.5179336
TATCAAC17600.049.0911252
ACGCAGA20550.041.336327
GGTATCA7050.039.829961
CGCAGAG21500.039.509838
GCAGAGT27450.030.9457689
TATACTG19950.027.0677875
GTACATG29600.026.0271421
ATTATAC19650.025.6489663
ATACCGT1701.082317E-425.4076186
TACATGG30100.025.3555932
ACATGGG29700.024.727383
GTATTAG5250.024.685821
ATACTGC19050.024.562926
TCTACAC13900.024.345433
TTATACT21800.024.1101974
Produced by FastQC (version 0.11.5)