FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817137
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTCGG39970.4891468627659744No Hit
ACATATTACTCTGGTAATAGAGGGTATTACTTTGACTACTGGGGCCAGGG29790.3645655502076151No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29770.3643207932084828No Hit
GTCTATTACTGTGCGACTGGGGGCCCTACATATTACTCTGGTAATAGAGG26350.32246734635685326No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA22950.28085865650435604No Hit
GGTAATAGAGGGTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC22130.2708236195399303No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20490.25075354561107865No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG19890.24341083563710858No Hit
GGCTAATATGTTCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA19300.23619050416270465No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19000.2325191491757196No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18770.22970444368569776No Hit
GTAATATGTAGGGCCCCCAGTCGCACAGTAATAGACGGCCGTGTCCTCAG18500.22640022419741118No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18460.22591071019914655No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18390.22505406070218334No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18200.22272886921042614No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18110.22162746271433062No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16850.20620777176899346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16830.2059630147698611No Hit
TCCCTATCTTTGGTACAACAAACTACGCACAGAAATTCCAGGGCAGAGTC16560.20265879528157457No Hit
ATATTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCT14530.17781595986964244No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG14250.17438936188178972No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13740.16814805840391514No Hit
CTATTACCAGAGTAATATGTAGGGCCCCCAGTCGCACAGTAATAGACGGC13730.16802567990434897No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13120.1605605914308127No Hit
ATATTACTCTGGTAATAGAGGGTATTACTTTGACTACTGGGGCCAGGGAA12840.15713399344295997No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12830.15701161494339383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12340.15101506846465157No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11650.142570951994586No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11550.1413471669989243No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11460.14024576050282878No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCG11440.14000100350369643No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11260.1377981905115054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11260.1377981905115054No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11160.13657440551584374No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA11150.13645202701627757No Hit
GAGTAATATGTAGGGCCCCCAGTCGCACAGTAATAGACGGCCGTGTCCTC11100.1358401345184467No Hit
GGCTATTAGCAACTGGTTAGCCTGGTATCAGCAGAGACCAGGTAAAGCCC11020.13486110652191738No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAA10840.13265829352972636No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10800.13216877953146167No Hit
CATAACAACCACAGTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT10630.13008834503883682No Hit
GTATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG10470.12813028904577814No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10210.12494844805705776No Hit
GGTATCAGCAGAGACCAGGTAAAGCCCCTAAGCTCCTGATCTATGCTGCA10190.1247036910579254No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10190.1247036910579254No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10080.12335752756269756No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGACCATCCCTATCTTTGGT9660.11821763058091848No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9600.11748335958352149No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9570.11711622408482299No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9530.11662671008655831No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9510.11638195308742597No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9430.11540292509089664No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCCACAGG9350.11442389709436727No Hit
AAGTAATACCCTCTATTACCAGAGTAATATGTAGGGCCCCCAGTCGCACA9130.11173157010391159No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9000.11014064960955139No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8960.10965113561128671No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT8820.10793783661736038No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8820.10793783661736038No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8770.10732594411952953No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8760.10720356561996336No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8760.10720356561996336No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8700.10646929462256635No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8650.1058574021247355No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8630.10561264512560317No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8600.10524550962690467No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8590.1051231311273385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8500.10402172463124298No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8490.10389934613167683No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8310.10169653313948579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.074.211631
TATCAAC16600.060.3317152
ATCAACG16750.058.9120033
TCAACGC16950.058.202624
CAACGCA17150.057.940725
AACGCAG18350.054.1483576
CGCAGAG20250.049.0677768
GAATACG1650.047.641179
CAGCGCT4950.046.19759
GGCAGCG6050.040.1726157
GCAGCGC6250.037.743368
GTCTATT16300.032.496451
ACGCAGA30750.032.3129237
GTACATG19500.031.93571
GTACTAG900.003861925331.8133431
GTGCTAG2705.456968E-1231.8133411
TTAGGGT1602.094106E-631.2760034
TACATGG19650.030.9444032
ACATGGG19900.030.183233
GCTAGAG3201.8189894E-1229.0491163
Produced by FastQC (version 0.11.5)