FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133959
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35850.316148996568659No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28960.25538842233272985No Hit
TTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGGCC26600.2345763823912505No Hit
GTGTGTACCTAGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCC26290.23184259748368327No Hit
GTATGTGAGATCTCCACTAGCACTAATACTTGAGACCCACTCCAGCCCCT25590.2256695347891767No Hit
GGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCGA22750.20062453757146423No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19550.17240482239657695No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAAAACA18730.16517352038301208No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAG17770.15670760583054588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17290.1524746485543128No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17070.1505345431360393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16880.14885899754753038No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGA16790.14806531805823667No Hit
GTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCT16680.14709526534909992No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT16310.1438323607820036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16220.14303868129270988No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGTGA15960.14074582943475028No Hit
TTTATAAGGTTTCTAGCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC15880.1400403365553781No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15740.13880572401647678No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCC15650.13801204452718308No Hit
AGGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCG15280.13474913996008675No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15050.1327208479318917No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGT14580.12857607726558015No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA14410.12707690489691426No Hit
CTCCAGAGACAATTCCATGAACACGCTGTTTTTGCAAATGAACAGCCTGA14350.12654778523738514No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA14240.12557773252824836No Hit
TCTCTGGAGAGGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTGAG14210.1253131726984838No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13760.12134477525201529No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13630.1201983493230355No Hit
GGTATCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT13610.12002197610319246No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13530.11931648322382027No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13400.11817005729484045No Hit
GTCTGGGACCCCAGAGTCCCGGCTAGAAACCTTATAAATTAGGCGCCTTG13380.11799368407499741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.11720000458570372No Hit
CTTTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGG12460.10988051596221733No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12400.1093513963026882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12280.10829315698362992No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12260.10811678376378686No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC12260.10811678376378686No Hit
TTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC12250.10802859715386537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12090.10661761139512099No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11960.1054711854661412No Hit
GCCTGGGGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA11760.10370745326771073No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT11600.10229646750896637No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11580.10212009428912333No Hit
GATTTCACACTGACGATCAGCAGGGTGGAGGCTGAGGATGTTGGACTTTA11490.10132641479982962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11400.10053273531053593No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG11390.10044454870061441No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC11370.10026817548077135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC450.00990858847.9975938
GTATCAA41050.036.8507771
TCAACGC41400.035.304134
ATCAACG41900.035.0562253
CAACGCA42100.034.7171255
AACGCAG43050.034.118266
TATCAAC43850.033.8256872
CGCAGAG44350.032.9543768
CATGGGG17350.031.9536934
GCAGAGT48300.030.5574729
GTACATG46000.027.4044271
TACATGG46400.027.1562042
ATGGGGA13500.025.5998445
GAATACG3409.822543E-1125.4104889
AGGGGTG9050.024.6628325
ACGCAGA61450.024.0183227
GGATCGC1500.001555923.9998536
TACACGG1500.001555923.9998535
ATTAGAG5500.023.5645313
ATATTAG5000.023.051041
Produced by FastQC (version 0.11.5)