FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133959
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36300.3201173940151275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27280.240573071865914No Hit
GTGTGTACCTAGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCC26430.23307721002258458No Hit
TTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGGCC24570.21667450057718135No Hit
GTATGTGAGATCTCCACTAGCACTAATACTTGAGACCCACTCCAGCCCCT24140.21288247635055588No Hit
GGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCGA20440.18025343067959249No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19810.17469767425453653No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAAAACA18430.1625279220853664No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGA17680.1559139263412522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17670.15582573973133068No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17100.15079910296580387No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16480.14533153315066946No Hit
GTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCT16480.14533153315066946No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAG16020.14127494909427943No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGTGA16010.1411867624843579No Hit
TTTATAAGGTTTCTAGCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC15930.14048126960498572No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT15680.13827660435694764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15420.13598375249898806No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCC15200.13404364708071456No Hit
CTCCAGAGACAATTCCATGAACACGCTGTTTTTGCAAATGAACAGCCTGA15110.13324996759142085No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14970.13201535505251955No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGT14540.12822333082589404No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA14440.12734146472667884No Hit
AGGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCG14370.12672415845722818No Hit
TCTCTGGAGAGGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTGAG13870.12231482796115205No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13870.12231482796115205No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA13380.11799368407499741No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13360.11781731085515436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13200.11640632509641001No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13130.11578901882695934No Hit
GTCTGGGACCCCAGAGTCCCGGCTAGAAACCTTATAAATTAGGCGCCTTG13040.11499533933766566No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12540.1105860088415895No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12510.11032144901182495No Hit
GGTATCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT12470.10996870257213885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12230.10785222393402231No Hit
GCCTGGGGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA12120.10688217122488555No Hit
TTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC12030.10608849173559184No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT11870.1046775059768475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11840.10441294614708292No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11830.1043247595371614No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11730.10344289343794616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11680.10300196038833856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11640.10264921394865247No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC11620.10247284072880943No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11440.100885481750222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC29250.029.0591934
ATCAACG29550.028.7641773
GTATCAA30700.027.9237881
CAACGCA30800.027.5968025
TATCAAC31300.027.1559542
GAATACG2955.2386895E-1026.8441129
AACGCAG31850.026.6740676
GTCTTAT1907.520297E-626.5405671
TACATGG42150.026.317862
GTACATG42600.026.2112561
CATGGGG18550.026.0170654
CGCAGAG33050.025.9211278
ATAGGAC2551.01019396E-725.4231723
GCAGAGT35550.023.8957569
TCTACAC9250.023.3618353
GACTCGG10350.022.9556857
TAGGACC2552.864128E-622.5983754
TAGACGG11850.022.4911065
ACTCGGG10400.022.1510858
ACATGGG50650.021.9012413
Produced by FastQC (version 0.11.5)