FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693210
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20750.29933209272803335No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGGGTGCTG14050.20268028447368042No Hit
GTTATATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC13910.20066069445045512No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13000.18753335929949078No Hit
CTCCTGTACTGGAACCAGCAGTGGCGTTGGTTATTACAATTATGTCTCCT12980.18724484643903003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.1797435120670504No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC12330.17786817847405548No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11860.17108812625322775No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11590.16719320263700754No Hit
GATTATTACTGCAGCTCATATATAACTGGCAGCACCCATGTGGTATTCGG11310.16315402259055697No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC11240.1621442275789443No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10830.15622971393949883No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTACTGGAACCAGCAGTGGCGTT10700.15435438034650395No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10520.15175776460235715No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10170.14670878954429395No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9610.13863042945139278No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9460.13646658299793715No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG9080.13098483864918278No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8770.12651288931204108No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8670.1250703250097373No Hit
GGTTATTACAATTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCC8650.12478181214927657No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT8650.12478181214927657No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8160.11771324706798805No Hit
ATATATAACTGGCAGCACCCATGTGGTATTCGGCGGAGGGACCAAGCTGA8090.11670345205637542No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8050.1161264263354539No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC8010.11554940061453241No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7960.11482811846338051No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7940.11453960560291974No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7940.11453960560291974No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7930.11439534917268937No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7890.11381832345176786No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7850.11324129773084635No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGGGTGCTGC7830.11295278487038561No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7640.11021191269600843No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7430.10718252766117049No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT7380.10646124551001859No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG7370.10631698907978823No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7320.10559570692863635No Hit
GTCTGGAGGCCTCGGACACCGCCATTTATTTCTGTGCGAGACCCCCCGAC7120.10271057832402879No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7040.10155652688218578No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7010.10112375759149465No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT6950.10025821901011238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.068.922941
TCAACGC17450.056.9510734
ATCAACG17750.056.3942263
TTAGACG400.006213166354.0106623
CAACGCA18700.053.144185
TATCAAC19250.052.373972
AACGCAG19250.051.625776
GAATACG1950.047.9956259
ACGCAGA21500.046.209747
CGCAGAG21650.045.889588
CACCGGT855.5652992E-542.349087
GCAGAGT25000.039.740389
GTGCTAG3200.036.0279051
ACACCGG1001.4471154E-436.0071076
TATAGCG1251.324117E-534.5668224
ACGCAAC850.002833264433.889046
CGGCGTG900.003754171831.9970829
TAGACGT1153.2882186E-431.3105284
CAGAGTA23950.029.759299
GTCTATA2951.6370905E-1129.3108371
Produced by FastQC (version 0.11.5)