FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693210
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21650.3123151714487673No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGGGTGCTG14290.20614243879920946No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13190.19027423147386796No Hit
GTTATATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC13030.1879661285901819No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC12570.18133033279958455No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11960.17253069055553152No Hit
CTCCTGTACTGGAACCAGCAGTGGCGTTGGTTATTACAATTATGTCTCCT11900.17166515197414925No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC11720.16906853623000245No Hit
GATTATTACTGCAGCTCATATATAACTGGCAGCACCCATGTGGTATTCGG11000.15868207325341527No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10790.15565268821857733No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTACTGGAACCAGCAGTGGCGTT10780.15550843178834697No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10570.15247904675350904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10460.1508922260209749No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10160.14656453311406356No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9640.13906319874208395No Hit
ATATATAACTGGCAGCACCCATGTGGTATTCGGCGGAGGGACCAAGCTGA9530.13747637800954976No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9490.13689935228862826No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9130.13170612080033467No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8910.12853247933526638No Hit
GGTTATTACAATTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCC8900.128388222905036No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG8740.12608012002134997No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC8730.12593586359111958No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT8660.12492606857950693No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8320.1200213499516741No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8100.1168477084866058No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGGGTGCTGC8010.11554940061453241No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7930.11439534917268937No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7920.11425109274245901No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7760.11194298985877295No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7570.10920211768439578No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7370.10631698907978823No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7290.10516293763794521No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7200.10386462976587181No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7200.10386462976587181No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7190.10372037333564145No Hit
GTAATAACCAACGCCACTGCTGGTTCCAGTACAGGAGATGGTGATCGACT7150.10314334761471994No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7140.10299909118448955No Hit
GTCTGGAGGCCTCGGACACCGCCATTTATTTCTGTGCGAGACCCCCCGAC7130.10285483475425918No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7100.10242206546356805No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT7090.10227780903333766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)