FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312826
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT43770.3334029033550524No Hit
GTAATGGGTACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCT32450.24717670125363145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29900.22775295431382378No Hit
GTAATAGACGGCAGTGTCCTCAGCCTGTAGGCCATTGATCTGCAGATATG27490.20939560916678981No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25240.192257008925783No Hit
GTCTATTACTGTGCGAGGGTCCCCTATGACGATTTTTGGAGTGGTTATCC22170.16887234104138704No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG21290.16216924405823774No Hit
TTATTACTGCATGCAAGCCCTACAAACTTGGACGTTCGGCCAAGGGACCA21170.16125518537871736No Hit
GCATAGTAATGGGTACAACTATTTGGATTGGTACGTGCAGAAGCCAGGGC20760.1581321515570228No Hit
GATTTTATACTGGAAATCAGCAGCGTGGAGGCTGAGGATGTTGGGGTTTA20630.15714192132087573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19380.14762047674253861No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18860.14365955579795037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18360.13985097796661553No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACGCTGCTGATTTCCAGTATAA17830.1358138854654006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16530.12591158310393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15730.11981785857379423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15000.11425733494004536No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14850.11311476159064492No Hit
GTATAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG14750.11235304602437794No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14370.10945852687256345No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT14240.10846829663641641No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14160.10785892418340283No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG14120.10755423795689603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13440.10237457210628065No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13260.10100348408700009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)