FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103333
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA45960.41655601708641005No Hit
GCCCTATATTACTGTGCGAAGCTAGTGAGTACAGCTGGAACTACAAGTTG45890.41592157580712263No Hit
CTATATTACTGTGCGAAGCTAGTGAGTACAGCTGGAACTACAAGTTGGTT42650.38655600802296314No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACAGCG39710.3599094742928925No Hit
GTGTGAGGTGCGGCTAGTGGAGTCTGGAGGAGACTTGGTCCAGCCGGGGG38970.35320252362614No Hit
ATATTACTGTGCGAAGCTAGTGAGTACAGCTGGAACTACAAGTTGGTTCG33680.3052568898057069No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28420.2575831593906826No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC26710.2420846652823762No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25440.23057408778673344No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23950.21706955198475894No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23160.2099094289756583No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22620.2050151676782984No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22030.19966773403859037No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21670.19640489317368373No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACCAGTCAGGCTG21440.19432030039888232No Hit
CTCCTGCACTGGGAGCGCCTCCAACATCGGGGCAGGTTATGATGTAAACT19220.17419944839862492No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19190.173927544993216No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18930.1715710488130057No Hit
CTCCTGTGCAGTCTCTGGGTTTGACGTCAGTAGCAACTACATGAGTTGGG18700.16948645603820423No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17850.16178252621828587No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACC17600.15951666450654517No Hit
GACTACGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGACACACTTC17470.15833841641644No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17450.15815714747950074No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16970.1538066929929586No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16210.14691847338926686No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15580.14120850187568032No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15380.13939581250628777No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA15290.13858010229006112No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15000.1359517027044419No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14310.12969792438003758No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGGTTTGACGTCAGTAGCAACTAC14030.127160159262888No Hit
GTCTCTGGGTTTGACGTCAGTAGCAACTACATGAGTTGGGTCCGCCAGGC13960.1265257179836006No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGCTTCCACCAATCGGCCCT13910.12607254564125245No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13860.12561937329890432No Hit
AACACAGACTACGCAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGACA13800.12507556648808654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.12498493201961693No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13670.12389731839798138No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13650.12371604946104214No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13370.12117828434389255No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13360.12108764987542292No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG13320.12072511200154441No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACACCAGTCAGGCTGG13220.11981876731684814No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13120.11891242263215185No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13110.11882178816368222No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC13050.11827798135286445No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13020.11800607794745557No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12530.1135649889924438No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12510.11338372005550454No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12490.11320245111856529No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12360.11202420302846013No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA12300.11148039621764236No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12240.1109365894068246No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12220.11075532046988534No Hit
GGTTATGATGTAAACTGGTACCAACAGCTTCCAGGAACAGCCCCCAAACT12210.11066468600141571No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA12150.11012087919059793No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12070.10939580344284093No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11890.10776438301038761No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11880.10767374854191798No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11680.10586105917252543No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11660.10567979023558616No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11640.10549852129864692No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11630.1054078868301773No Hit
GAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTCT11610.10522661789323802No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11530.10450154214548102No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11290.10232631490220993No Hit
TTTAAAAGGTGCCCAGTGTGAGGTGCGGCTAGTGGAGTCTGGAGGAGACT11260.10205441149680106No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11090.10051362553281737No Hit
GTAATAATCAGCCTCATCCTCAGTCTGGAGCCCAGTGATGGCCAGGGAGG11040.10006045319046923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)