FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607659
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20200.33242328345338423No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19740.3248532482856339No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19230.31646038320834546No Hit
TTTAAGAGGAGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16970.27926847129722426No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16960.27910390531531665No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16190.26643232470843026No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15720.2586977235587723No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15320.2521150842824676No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGATGCCT14780.24322852125945638No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14670.2414182954584726No Hit
GTAATAAACGGCCGTGTCCTCAGGTGCCAGGCTGTTCATTTGCAGATACA14640.24092459751274975No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14590.24010176760321167No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13320.21920188790094441No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC11490.18908631321185074No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCCGATTATTATTGTTCCTCATAT11380.18727608741086696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.18513672964606795No Hit
AACAAGTACTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA10740.17674386456877952No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10240.16851556547339874No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10210.1680218675276759No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10140.16686990565432255No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10110.1663762077085997No Hit
GTTTATTACTGTTCCAGTGGCTTCAATTCTGGGCACTACTTTGACTACTG9960.1639077179799855No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9900.1629203220885398No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGATATT9810.16143922825137125No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGGATAGCAGAGGTAC9790.16111009628755601No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9730.16012270039611032No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9700.15962900245038747No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9690.15946443646847985No Hit
GTACTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT9660.158970738522757No Hit
ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCC9660.158970738522757No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9650.1588061725408494No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9520.1566668147760504No Hit
GGGCTGGAGTGGGTGGCGGTTATCTCATATGATGGAAGTAACAAGTACTA9370.15419832504743616No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9310.15321092915599044No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9280.1527172312102676No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9240.15205896728263713No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8980.14778025175303913No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8840.1454763280063325No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8690.14300783827771826No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTCAGTACCTCTGCTATCCACTGGG8660.14251414033199541No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAG8650.1423495743500878No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8480.13955195265765832No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8400.1382354248023974No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8380.13790629283858216No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8340.1372480289109517No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8240.13560236909187554No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8150.13412127525470702No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA8070.13280474739944606No Hit
GTATCTGCAAATGAACAGCCTGGCACCTGAGGACACGGCCGTTTATTACT8030.1321464834718156No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGATGCCTG7940.13066538963464708No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7920.13033625767083182No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7920.13033625767083182No Hit
GTACTTGTTACTTCCATCATATGAGATAACCGCCACCCACTCCAGCCCCT7880.12967799374320135No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTCCTCCACGGTGC7880.12967799374320135No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT7850.12918429579747853No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7840.1290197298155709No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC7810.12852603186984804No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7790.12819689990603284No Hit
CTCCTGCACTGGAAGCAGCAGTGATATTGGTGGTTATAATTATGTCTCCT7730.1272095040145871No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7660.1260575421412338No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7630.12556384419551098No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7600.12507014624978813No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7470.1229307884849891No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7400.12177882661163579No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG7370.12128512866591296No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA7250.11931033688302156No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7150.11766467706394541No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7030.115689885281054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7020.11552531929914639No Hit
CTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGG7010.11536075331723879No Hit
AGTCAAAGTAGTGCCCAGAATTGAAGCCACTGGAACAGTAATAAACGGCC6970.11470248938960832No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC6920.11387965948007023No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT6910.11371509349816261No Hit
GCCTGGCACCTGAGGACACGGCCGTTTATTACTGTTCCAGTGGCTTCAAT6880.11322139555243976No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6810.11206943367908648No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6800.11190486769717883No Hit
TGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCC6770.11141116975145599No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT6700.1102592078781027No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC6650.1094363779685646No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6620.10894268002284174No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6500.10696788823995036No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT6480.10663875627613514No Hit
GCGTAGTACTTGTTACTTCCATCATATGAGATAACCGCCACCCACTCCAG6470.10647419029422751No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTCCTCCACGGTGCTCCCTTCA6440.10598049234850468No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC6400.1053222284208742No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA6370.10482853047515137No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCCCCTTCAGTACCTCTGCT6330.1041702665475209No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA6320.10400570056561327No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT6280.1033474366379828No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT6210.1021954747646295No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6140.10104351289127618No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6120.10071438092746096No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA6100.10038524896364572No Hit
CTGTAGGGGCCACTGTCTCCTCCACGGTGCTCCCTTCATGCGTGACCTGG6090.10022068298173811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15750.062.2355231
ATCAACG19250.050.49983
TATCAAC19400.050.1093372
TCAACGC19450.049.972294
CAACGCA19900.048.8422665
AACGCAG20350.048.1160136
GCAGCGC4350.046.339168
CGCAGAG21950.044.6050268
CAGCGCT4400.044.1764149
GGCAGCG4500.043.1947177
GCAGAGT24200.040.4578639
CCTATAT1157.478793E-637.56993
GAATACG1201.0030428E-535.9955989
GGTATCA8850.033.3903161
AGCGCTG4750.033.3460355
ATTACAC1751.0700933E-732.918393
ACTAGAC950.004885563630.3195693
TATTACA1952.7641727E-729.5421432
ACGAGTG2701.8553692E-1029.332165
GCGCTGG5450.029.0630576
Produced by FastQC (version 0.11.5)