FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607659
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25000.41141495476903983No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19450.320080834810313No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18570.30559902840244285No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16250.2674197205998759No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15990.2631410050702779No Hit
TTTAAGAGGAGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG15830.2605079493597561No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15580.25639379981206567No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14900.24520331304234777No Hit
GTAATAAACGGCCGTGTCCTCAGGTGCCAGGCTGTTCATTTGCAGATACA14750.24273482331373353No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGATGCCT14440.23763327787459745No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14370.2364813160012441No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13570.22331603744863482No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12000.19747917828913916No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11920.19616265043387823No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC11890.19566895248815538No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11830.19468155659670966No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11340.1866178234832365No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11310.18612412553751365No Hit
TCTCTGGACTCCAGGCTGAGGATGAGGCCGATTATTATTGTTCCTCATAT10900.17937692027930138No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10530.1732879789487196No Hit
GTTTATTACTGTTCCAGTGGCTTCAATTCTGGGCACTACTTTGACTACTG10080.16588250976287688No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9830.16176836021518648No Hit
AACAAGTACTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA9670.15913530450466462No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9670.15913530450466462No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTCAGTACCTCTGCTATCCACTGGG9610.15814790861321892No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGATATT9320.15337549513789805No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9260.15238809924645239No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9220.1517298353188219No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGGATAGCAGAGGTAC9190.15123613737309907No Hit
GTACTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT9020.1484385156806696No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9000.14810938371685436No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8970.14761568577113152No Hit
ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCC8880.14613459193396294No Hit
GGGCTGGAGTGGGTGGCGGTTATCTCATATGATGGAAGTAACAAGTACTA8880.14613459193396294No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAG8830.1453117620244249No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8600.14152674444054972No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8570.14103304649482687No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8550.14070391453101164No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8520.1402102165852888No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG8450.13905825471193548No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8380.13790629283858216No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8260.1359315010556908No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8260.1359315010556908No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT8210.1351086711461527No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8180.13461497320042984No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7930.13050082365273946No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7900.1300071257070166No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7870.12951342776129374No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGATGCCTG7830.1288551638336633No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7780.12803233392412522No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTCCTCCACGGTGC7740.12737406999649475No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC7690.12655124008695667No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7670.12622210812314144No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7650.1258929761593262No Hit
GTACTTGTTACTTCCATCATATGAGATAACCGCCACCCACTCCAGCCCCT7650.1258929761593262No Hit
GTATCTGCAAATGAACAGCCTGGCACCTGAGGACACGGCCGTTTATTACT7470.1229307884849891No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7450.12260165652117389No Hit
CTCCTGCACTGGAAGCAGCAGTGATATTGGTGGTTATAATTATGTCTCCT7430.12227252455735864No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT7310.12029773277446726No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7310.12029773277446726No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7300.12013316679255964No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7270.1196394688468368No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7240.11914577090111395No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7200.11848750697348348No Hit
AGTCAAAGTAGTGCCCAGAATTGAAGCCACTGGAACAGTAATAAACGGCC7170.11799380902776063No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7130.11733554510013017No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7060.11618358322677685No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6940.11420879144388547No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG6860.11289226358862454No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG6790.11174030171527123No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6790.11174030171527123No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6660.10960094395047221No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT6620.10894268002284174No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA6600.10861354805902652No Hit
CTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGG6570.10811985011330368No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6500.10696788823995036No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC6450.1061450583304123No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6400.1053222284208742No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA6370.10482853047515137No Hit
GCCTGGCACCTGAGGACACGGCCGTTTATTACTGTTCCAGTGGCTTCAAT6350.10449939851133612No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC6330.1041702665475209No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT6270.1031828706560752No Hit
TGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCC6260.10301830467416759No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6190.10186634280081427No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6140.10104351289127618No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG6100.10038524896364572No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT6080.1000561169998305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC10550.048.49312
TCAACGC10400.047.1061974
ATCAACG10450.046.884673
GTATCAA11250.046.760611
GAATACG1454.110916E-1044.684329
CAACGCA11150.043.9345
AACGCAG11200.043.712666
CGCAGAG11500.042.5723348
GGCAGCG3250.039.9082952
GTGCTAG1701.9390427E-938.150781
GTACACG8100.037.3534745
TACACGG8100.037.3319446
TATGTAC8150.037.1334842
GTATGTA8750.037.0607571
GCAGAGT13450.036.3971379
TATTGAG6350.036.3030365
ACACGGG8450.035.785657
ACGGGGT8500.035.572229
GCAGCGC3650.035.5318573
ATGTACA8900.034.0014573
Produced by FastQC (version 0.11.5)