FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22200.272930126199448No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAACGTCTGGGGCCC20080.24686652856238359No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAACGTCT19610.24108827814284572No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCA19020.23383472974385142No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCT17060.20973819607939562No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15550.19117402983790163No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15550.19117402983790163No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13500.1659710226888535No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTCTAGGAATAATCAGCGGCCCT13070.1606845382624678No Hit
GTGCTGACTCAGCCACCCGCAACGTCTGGGGCCCCCGGGCAGACGATCAC12880.15834864979499505No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12790.1572421763104027No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12570.15453746334806584No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA12170.14961980341654424No Hit
GAAATAGTCAGCCTCATCCTCGGACCGGAGTCCACCGATGGCCAGGGACG12160.1494968619182562No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11230.13806330257746852No Hit
GTTTAGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11070.13609623860485986No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10750.13216211065964262No Hit
GTAATACACGGCCGTGTCTTCGGATCTCAGGCTGCTCAGCTCCATGTAGG10720.1317932861647785No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10680.13130152017162633No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT10610.13044092968361004No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10480.12884269020586556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10400.12785915821956123No Hit
GACTATTTCTGTGCAACATGGGATGACAGCCTGCGTGCTTGGGTGTTCGG10230.12576915274866454No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG10170.12503150375893632No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9510.11691736487192571No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9380.11531912539418118No Hit
ACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCAAGCACGCAGGC9260.11384382741472471No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9230.1134750029198606No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9220.11335206142157254No Hit
GTATATTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTC9190.11298323692670842No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9150.11249147093355626No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT9110.11199970494040412No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8420.10351674155852936No Hit
GACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCAAGCACGCAGG8360.10277909256880113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.1026561510705131No Hit
GTGTATTACTGTGTGGCAGACGCCCAAGGATTTGGAATGGTTACCAAATC8280.10179556058249682No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8190.10068908709790446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16350.065.25821
GGTATCA5850.064.082371
TCAACGC16700.061.258864
ATCAACG17100.060.668533
CAACGCA17550.058.7024085
AACGCAG18150.056.7129866
TATCAAC18550.055.537862
ACGCAGA21100.048.780927
CGCAGAG21150.048.66568
GAATACG2400.041.9868439
GCAGAGT25400.040.2393539
GTGCTAG3950.036.5026131
GTACATG21500.034.872731
GTCTTAG3000.031.2401561
ATACCGT1050.00798213527.4216636
ATGGGAG11550.026.8215275
CAGAGTA25200.025.9918549
TACATGG29100.025.5000652
GTGCGGA1500.0015438624.030891
CCCAATA2401.6876565E-624.0146262
Produced by FastQC (version 0.11.5)