FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAACGTCTGGGGCCC19620.2412112196411338No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19100.2348182617301557No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCGCAACGTCT18240.2242452928773843No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCA17540.21563938799722152No Hit
CTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCT15900.19547698227798302No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15450.18994461485502123No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14190.17445398607072823No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTCTAGGAATAATCAGCGGCCCT12050.14814450543708776No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11900.14630038296276718No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11690.14371861149871834No Hit
GTGCTGACTCAGCCACCCGCAACGTCTGGGGCCCCCGGGCAGACGATCAC11480.1411368400346695No Hit
GTAATACACGGCCGTGTCTTCGGATCTCAGGCTGCTCAGCTCCATGTAGG11360.13966154205521303No Hit
GAAATAGTCAGCCTCATCCTCGGACCGGAGTCCACCGATGGCCAGGGACG11190.13757153658431634No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT10860.13351446714081106No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10480.12884269020586556No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10410.12798209971784927No Hit
CCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10350.12724445072812102No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10140.1246626792640722No Hit
GTTTAGAACCTATGAACACTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10110.12429385476920808No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9800.12048266832227884No Hit
GACTATTTCTGTGCAACATGGGATGACAGCCTGCGTGCTTGGGTGTTCGG9720.11949913633597453No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA9490.1166714818753496No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9420.11581089138733333No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9090.11175382194382802No Hit
ACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCAAGCACGCAGGC8870.10904910898149117No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8860.10892616748320312No Hit
GACCCAGGACGGTCACCTGGGTCCCTCCGCCGAACACCCAAGCACGCAGG8440.10376262455510546No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8340.10253320957222506No Hit
GTATATTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTC8330.10241026807393701No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8240.10130379458934466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA10550.055.9069445
TCAACGC10450.055.7433324
ATCAACG10950.052.5412063
AACGCAG11250.052.428296
GGTATCA5100.050.8360331
GTATCAA11800.050.046211
ACGCAGA12900.045.1647577
TATCAAC12950.044.982072
CGCAGAG13150.044.3088388
GAATACG2600.044.266769
TGTACCG1252.8920658E-740.2802735
GTACATG20000.038.529481
TACGCTA850.002849172633.851059
GCAGAGT17150.033.135549
TCTACAC6950.032.077543
ACGGCGT1153.310413E-431.2754298
GTGCTAG4350.028.1448551
TACATGG28650.027.8626562
CATGGGG12500.027.615664
ATGGGAG9550.026.3614355
Produced by FastQC (version 0.11.5)