FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716785
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGTACTATGGTGACTCGCCTCACACTTTTGGCCAGGGGA19490.27190859183716176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16330.22782284785535412No Hit
ACATAGACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCAAGA12400.17299469157418193No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGGCTGTTGATGGTGACGGT12220.17048347830939542No Hit
GTACTATGGTGACTCGCCTCACACTTTTGGCCAGGGGACCAGGCTCGAGG12070.16839080058874No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11530.16085716079438048No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCGTCTCCT10560.14732451153414206No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10430.145510857509574No Hit
CCATAGTACTGACAATAATACACTGCAAAATCTTCAGGCTCCAGGCTGTT9190.12821138835215581No Hit
TCCTTAGCCTGGTATCAACATAGACCTGGCCAGCCTCCCAGGCTCCTCAT8550.11928263007735933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8230.11481825093996108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7420.10351779124842177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7230.10086706613559157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11950.060.1562084
ATCAACG12000.059.901373
AACGCAG12500.058.100656
CAACGCA12500.057.517365
ACGCAGA13850.051.9218567
CGCAGAG14850.048.4254348
ACACCGT750.001544207838.3502666
GCAGAGT19100.037.6501439
GTATCAA22750.036.4043921
TATCAAC26300.030.6111972
GTACATG21750.030.1314091
TACATGG22600.028.3073752
GTATAGA3900.027.6989941
ACATGGG24050.026.0029243
GTGTATG5600.025.7204911
GTATTAT14300.025.684521
TATTGTC12900.023.4082285
TAGGACG1851.938409E-423.3145964
ATACGAG3554.5474735E-922.2731883
TCTACAC5550.022.0178033
Produced by FastQC (version 0.11.5)