FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754995
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCCGCCTCATCCTCGGACCGGAGTCCACTGATGGCCAGGGAGG37980.5030496890707886No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34140.4521884250889079No Hit
ACCTAGGACAGTCACCGTGGTCCCTCCGCCGAACACCCAATGGCCACTCG26670.3532473724991556No Hit
GTAATACACGGCCGTGTCGTCGGTTCTCAGGCTGCTCAGCTCCATGTAGG24900.3298035086325075No Hit
GATTATTACTGTGCGACATGGGAAGACAGCCCGAGTGGCCATTGGGTGTT24710.3272869356750707No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGCGACCC16930.22423989562844787No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16890.22371009079530327No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT16790.2223855787124418No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16600.21986900575500498No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15330.20304770230266422No Hit
GTGTATTACTGTGCGGCAGATCTCTCTTTTGGAGTCGTTACAAGTACGTC14760.19549798343035385No Hit
GCCTTGGGCTGACCTAGGACAGTCACCGTGGTCCCTCCGCCGAACACCCA14610.19351121530606163No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13920.18437208193431745No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13810.1829151186431698No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13680.1811932529354499No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13370.17708726547857934No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13240.1753653997708594No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13170.17443824131285637No Hit
GTAGATATACGTTGCAGTGGGTGCGACAGGCTCGTGGACAGCGCCTTGAG13010.17231902198027801No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG12920.1711269611057027No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12910.17099450989741655No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12720.16847793693997973No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC12420.16450440069139532No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12300.16291498619196154No Hit
GTCTGGGCCTGAGGTGAAGAAGCCGGGGACCTCAGTGAAGGTCTCCTGCA11930.15801429148537408No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11930.15801429148537408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11610.15377585282021736No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11410.15112682865449442No Hit
TCCTATTACTACCTTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT11400.15099437744620825No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11330.15006721898820521No Hit
GCCTAAGTAGATATACGTTGCAGTGGGTGCGACAGGCTCGTGGACAGCGC11320.14993476777991907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.14728574361419614No Hit
GTCTTCCCATGTCGCACAGTAATAATCCGCCTCATCCTCGGACCGGAGTC10970.14529897548990392No Hit
CTCTAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT10930.14476917065675932No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10930.14476917065675932No Hit
TTCCTAGAGATGAGTAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCA10650.1410605368247472No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10370.13735190299273503No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA9850.13046444016185538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA9810.1299346353287108No Hit
CTCGTGGACAGCGCCTTGAGTGGATAGGATGGATCGTCGTTGGCAGTGGT9540.1263584527049848No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9490.12569619666355408No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9400.12450413578897873No Hit
TTATTACTGTGCGACATGGGAAGACAGCCCGAGTGGCCATTGGGTGTTCG9360.12397433095583414No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9320.12344452612268957No Hit
GTAATAGGACGTACTTGTAACGACTCCAAAAGAGAGATCTGCCGCACAGT9280.12291472128954496No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9270.12278227008125882No Hit
GACCTAGGACAGTCACCGTGGTCCCTCCGCCGAACACCCAATGGCCACTC9260.12264981887297266No Hit
TGGGAAGACAGCCCGAGTGGCCATTGGGTGTTCGGCGGAGGGACCACGGT9150.12119285558182505No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9050.11986834349896358No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9040.11973589229067741No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8850.11721931933324063No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8800.11655706329180988No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8750.11589480725037915No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8660.11470274637580381No Hit
CCCAATGGCCACTCGGGCTGTCTTCCCATGTCGCACAGTAATAATCCGCC8630.11430539275094538No Hit
CAGTAATACACGGCCGTGTCGTCGGTTCTCAGGCTGCTCAGCTCCATGTA8620.11417294154265922No Hit
GTATTACTGTGCGGCAGATCTCTCTTTTGGAGTCGTTACAAGTACGTCCT8410.11139146616865013No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8380.11099411254379167No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8250.10927224683607176No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8220.10887489321121332No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8180.10834508837806872No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT8120.10755038112835184No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8080.10702057629520725No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8040.10649077146206266No Hit
GCCTACATGGAGCTGAGCAGCCTGAGAACCGACGACACGGCCGTGTATTA8000.10596096662891807No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7920.1049013569626289No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT7910.10476890575434274No Hit
ATACACGGCCGTGTCGTCGGTTCTCAGGCTGCTCAGCTCCATGTAGGCTG7900.10463645454605659No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT7870.10423910092119816No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7810.10344439367148126No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAAGTACGCACAGACG7740.10251723521347822No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7690.10185497917204749No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7690.10185497917204749No Hit
CAGTAATAATCCGCCTCATCCTCGGACCGGAGTCCACTGATGGCCAGGGA7610.1007953695057583No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7580.10039801588089987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.068.559991
GGTATCA7050.062.344151
ATCAACG17800.062.2971233
TCAACGC17950.061.768354
TATCAAC18750.059.5247652
CAACGCA18650.059.4499665
AACGCAG19200.057.746976
TTTCGCG502.0804032E-457.5969779
TACCGGT400.00621952553.997174
CGCAGAG22450.049.3871658
GAATACG1700.046.5857969
GCAGAGT24700.045.1798179
TCTAGAC1202.0814514E-742.0033653
ACGCAGA27800.039.8828057
GTGCTAG2450.038.2324641
AATCCGC22050.034.6104286
TAATCCG22250.033.9757425
ATCCGCC23550.032.4059457
TCCGCCT23500.032.1685268
GTACATG22050.031.6971
Produced by FastQC (version 0.11.5)