FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624291
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGGTTGGAGATGGTGAGGTAGC11630.18629132888348543No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11190.17924333363767858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.15681789421920228No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGTGTTACTG9780.1566577125090703No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG9660.1547355319874866No Hit
CTCCCAGCCTGTGCTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGAT9520.152492988045639No Hit
ATGTATTACTGTGCGAGACGGGGCGGTGACTTCCCCTTCATCTACGGTAT7710.12350009851175173No Hit
GATTATTACTGTGAGACCTGGGACAGTAACACTCAGGTGTTCGGCGGAGG7560.12109737285977212No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG7540.12077700943950817No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT6330.10139502251353937No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6290.10075429567301146No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT605.117036E-447.996616
GTATCAA18950.047.8891451
TCAACGC21300.042.588544
CAACGCA21450.042.2907185
ATCAACG21350.042.151593
AACGCAG22200.040.861986
TATCAAC22900.039.612922
CGCAGAG24750.036.6519558
ACGCAGA26150.034.689717
GCAGAGT28450.031.885279
GTACACG3600.027.998025
TACACGG3750.026.87816
GTATGTA6050.026.1904581
ACGGGGT4200.025.712479
GTCCTAC5050.025.6718351
GTATTAG3251.6007107E-924.3772741
TATTGAG5350.024.2225885
ACACGGG4201.8189894E-1223.9983047
TGTACTA2903.510686E-722.3432485
GTAATAC7500.022.0872861
Produced by FastQC (version 0.11.5)