FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396990
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATACA29710.7483815713242147No Hit
GTGTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGCCGA19370.4879216101161239No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCG14990.37759137509760954No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10460.2634827073729817No Hit
ATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT10330.26020806569434995No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10280.2589485881256455No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10000.2518955137409003No Hit
GTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATACAGCG9990.25164361822715936No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9850.24811708103478677No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG9370.23602609637522354No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9030.22746164890803294No Hit
GTGTTGGAAGTATTCGGCGTTGACCACCACCTGAGGCATGTCGTGACTCG8930.22494269377062395No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8750.22040857452328774No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8670.21839341041336052No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8440.21259981359731983No Hit
ACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8200.2065543212675382No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7770.1957228141766795No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7410.1866545756820071No Hit
GTATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG7400.1864026801682662No Hit
CAGTAGTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATA7380.1858988891407844No Hit
GTACTTCTACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG7370.1856469936270435No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7350.18514320259956168No Hit
CTCCTGTGCAGTCTCTGGATTAACCTTCAGTACTTCTACTATGCACTGGG7190.1811128743797073No Hit
GAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTCCTCGT6690.16851809869266227No Hit
GCTGTGTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGC6660.16776241215143958No Hit
GTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGCCGAAT6530.16448777047280788No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACAGAAGGTCTT6520.16423587495906697No Hit
AATCAATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA6430.1619688153353989No Hit
GCGTAATATTGATTACTCCCATCATATGGTATAACTGCCACCCACTCCAG6400.16121312879417618No Hit
CTTCCAACACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA6370.16045744225295347No Hit
GTAGAAGTACTGAAGGTTAATCCAGAGACTGCACAGGAGAGTCTCAGGGA6180.15567142749187635No Hit
ATATTGATTACTCCCATCATATGGTATAACTGCCACCCACTCCAGCCCCT6130.15441194992317187No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT6060.15264868132698556No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5960.15012972618957657No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGTAGAAGTAC5870.14786266656590846No Hit
GGGCTGGAGTGGGTGGCAGTTATACCATATGATGGGAGTAATCAATATTA5840.14710698002468578No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA5770.14534371142849944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.14534371142849944No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5690.14332854731857225No Hit
CAGTTATACCATATGATGGGAGTAATCAATATTACGCAGACTCCGTGGAG5620.14156527872238595No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTC5590.14080959218116326No Hit
GTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCGTGGTCCAGCC5580.14055769666742235No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT5560.14005390563994055No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT5540.13955011461245875No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG5540.13955011461245875No Hit
GTCTCTGGATTAACCTTCAGTACTTCTACTATGCACTGGGTCCGCCAGGC5530.13929821909871787No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5280.13300083125519535No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCAC5260.13249704022771355No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG5220.13148945817274993No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG5170.13022998060404545No Hit
GTCTTAGGCTGGAGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCA5020.12645154789793195No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT5010.12619965238419104No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTAACCTTCAGTACTTCTACT4970.12519207032922744No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC4920.12393259276052293No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA4890.12317690621930025No Hit
GATCAGCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGG4890.12317690621930025No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT4860.12242121967807754No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG4830.12166553313685484No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAATCTGCTGGTA4730.11914657799944584No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA4730.11914657799944584No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4730.11914657799944584No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG4720.11889468248570494No Hit
GTATCTGCAAATGAATAGCCTGAGATTTGAGGACACGGCTGTGTACTACT4700.11839089145822314No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC4700.11839089145822314No Hit
CTACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG4680.11788710043074133No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC4640.11687951837577773No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA4510.11360487669714603No Hit
CTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAATAGCCTGA4470.11259729464218242No Hit
GTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA4430.11158971258721881No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG4420.11133781707347791No Hit
CTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGG4350.10957454847729162No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC4280.10781127988110531No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT4260.10730748885362351No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT4250.10705559333988263No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT4250.10705559333988263No Hit
TGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCC4210.10604801128491902No Hit
GGTTATGATGTGCACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACT4140.10428474268873271No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG4120.10378095166125091No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA4120.10378095166125091No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4100.1032771606337691No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTAACCTTCA4090.10302526512002823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9500.044.7610971
GAATACG1252.867564E-740.3150989
GTGTAGA1452.0256266E-839.7643851
TATCAAC10550.039.5880972
ATCAACG10750.038.8515783
CAACGCA10450.038.58395
TAGAATC750.001533547538.400174
ATACTAC951.06414635E-437.9331321
GTACACG5200.037.384785
ACGGGGT5200.037.3800749
TATGTAC5400.037.33822
AACGCAG11050.036.4888466
GGTATCA5200.036.0364761
TACACGG5450.035.669886
ACACGGG5550.035.022777
GTATGTA5600.034.749461
GTAGTAC13700.034.7212751
AGTACAC13150.034.4987953
TATACTA1051.9285065E-434.2858665
CAGCCGT13150.033.942649
Produced by FastQC (version 0.11.5)