FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396990
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATACA30140.7592130784150735No Hit
GTGTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGCCGA19330.48691402806116024No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCG14200.3576916295120784No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12820.32293004861583413No Hit
GTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATACAGCG10410.2622232298042772No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10340.2604599612080909No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9900.24937655860349126No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9680.24383485730119148No Hit
ATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT9670.24358296178745054No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG9580.24131590216378246No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9320.23476661880651906No Hit
GTTCCAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGACCTCAGTGTC9120.22972870853170108No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8930.22494269377062395No Hit
GTGTTGGAAGTATTCGGCGTTGACCACCACCTGAGGCATGTCGTGACTCG8870.22343132068817856No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7920.199501246882793No Hit
GTATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG7920.199501246882793No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7910.1992493513690521No Hit
GTACTTCTACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG7770.1957228141766795No Hit
ACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC7350.18514320259956168No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7350.18514320259956168No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7230.1821204564346709No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7200.18136476989344819No Hit
CAGTAGTACACAGCCGTGTCCTCAAATCTCAGGCTATTCATTTGCAGATA7090.1785939192422983No Hit
CTCCTGTGCAGTCTCTGGATTAACCTTCAGTACTTCTACTATGCACTGGG6900.1738079044812212No Hit
GAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTCCTCGT6710.1690218897201441No Hit
GCTGTGTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGC6410.16146502430791707No Hit
GTACTACTGTGCGAGTCACGACATGCCTCAGGTGGTGGTCAACGCCGAAT6410.16146502430791707No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGTAGAAGTAC6380.16070933776669438No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6290.15844227814302628No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6210.15642711403309906No Hit
AATCAATATTACGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA6140.15466384543691278No Hit
GCGTAATATTGATTACTCCCATCATATGGTATAACTGCCACCCACTCCAG6130.15441194992317187No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5960.15012972618957657No Hit
GTAGAAGTACTGAAGGTTAATCCAGAGACTGCACAGGAGAGTCTCAGGGA5920.14912214413461297No Hit
ATATTGATTACTCCCATCATATGGTATAACTGCCACCCACTCCAGCCCCT5900.14861835310713115No Hit
GGGCTGGAGTGGGTGGCAGTTATACCATATGATGGGAGTAATCAATATTA5770.14534371142849944No Hit
GTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCGTGGTCCAGCC5770.14534371142849944No Hit
CTTCCAACACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCA5670.14282475629109045No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5600.14106148769490415No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT5550.13980201012619964No Hit
CAGTTATACCATATGATGGGAGTAATCAATATTACGCAGACTCCGTGGAG5500.13854253255749516No Hit
GTCTCTGGATTAACCTTCAGTACTTCTACTATGCACTGGGTCCGCCAGGC5470.13778684601627245No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT5400.13602357742008614No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT5360.13501599536512254No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG5310.13375651779641806No Hit
GATCAGCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGG5160.12997808509030453No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG5140.12947429406282274No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTC5100.12846671200785914No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC5080.12796292098037734No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5050.12720723443915466No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG5050.12720723443915466No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA5010.12619965238419104No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA4980.12544396584296832No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACAGAAGGTCTT4920.12393259276052293No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4910.12368069724678203No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT4910.12368069724678203No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG4850.12216932416433664No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTAACCTTCAGTACTTCTACT4820.12141363762311394No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG4750.11965036902692762No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG4720.11889468248570494No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT4670.11763520491700043No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC4580.11536814529333232No Hit
CTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAATAGCCTGA4540.11436056323836873No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC4520.11385677221088693No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCAC4370.11007833950477343No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG4350.10957454847729162No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGACCTCAGTGTCCGGGGCCC4300.10831507090858711No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC4290.10806317539484621No Hit
TGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAATCGGCC4210.10604801128491902No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT4200.10579611577117812No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA4200.10579611577117812No Hit
CTACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG4190.10554422025743722No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA4190.10554422025743722No Hit
CTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGG4180.10529232474369632No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC4170.10504042922995542No Hit
GTATCTGCAAATGAATAGCCTGAGATTTGAGGACACGGCTGTGTACTACT4170.10504042922995542No Hit
GGTTATGATGTGCACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACT4160.10478853371621451No Hit
GTGCATAGTAGAAGTACTGAAGGTTAATCCAGAGACTGCACAGGAGAGTC4050.10201768306506462No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC4040.10176578755132372No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT4010.10101010101010101No Hit
GTGTCAGGTGCAGCTGGTGCAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA4010.10101010101010101No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT4010.10101010101010101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA400.006220698453.9898389
TTAGTAC450.00986985948.039413
ACGGGTT450.0098994148.003078
GGCAGCG2350.046.0067862
GCGCTGG2700.042.669396
GAATACG1351.08957465E-842.6586389
GTATCAA7650.041.4562231
GCTGGGT2800.041.1454858
GCAGCGC2650.040.7881773
GCTAGAG1452.0290827E-839.7567523
TATCAAC8050.038.5009162
ATCAACG8050.038.4912033
AGCGCTG3300.037.1213575
CAACGCA8350.037.1082845
GTGCTAG1604.8194124E-836.0386471
ATGGGGC2001.8189894E-1136.0295525
AACGCAG8650.035.7941936
CAGCGCT3250.035.4752544
ACATGGG12600.034.885763
GTACATG12850.034.215681
Produced by FastQC (version 0.11.5)