FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950054
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAGACGGCCGTGTCGTCAGATCTCAGGTTCCTCAGTTCCAGGTAGG34310.3611373669286167No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25260.2658796236845484No Hit
GTCTGGAACTGTGGTGGAGAGGCCTGGGGCCTCAGTGAGGGTCTCCTGCA24020.25282773400248826No Hit
GTCTATTATTGTGCGATGAGTTCTGAGGTGACGATTTTTCACGTGGGCCG19130.20135697549823486No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19080.20083068962395823No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17270.18177914097514458No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16270.17125342348961217No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15740.16567479322228No Hit
CCTCAGAACTCATCGCACAATAATAGACGGCCGTGTCGTCAGATCTCAGG15430.16241182080176494No Hit
GTGGTGGAGAGGCCTGGGGCCTCAGTGAGGGTCTCCTGCAAGCCTTCTGG15350.16156976340292237No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15230.16030667730465847No Hit
GGATGGAACAGCGCTTACAATGGTGACACAAACTATGCACAGAGGTTCCA14690.15462278986247097No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14100.14841261654600685No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13860.1458864443494791No Hit
GTTCCATCCCATCCACTCAAGGCCTTGTCCAGGGGCCTGTCGCACCCAGT13750.14472861542607052No Hit
CAATAATAGACGGCCGTGTCGTCAGATCTCAGGTTCCTCAGTTCCAGGTA13380.14083409995642354No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13350.14051832843185755No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12740.1340976407656828No Hit
GTTCTGAGGTGACGATTTTTCACGTGGGCCGTGGGAGGGGCCGATTCGAC12270.12915055354748256No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12040.1267296385258101No Hit
GTCCAGGGGCCTGTCGCACCCAGTTGAAAGCATAGTTGGTAAAGCTGTAA11760.12378243762986103No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11270.11862483606195016No Hit
CCCCTGGACAAGGCCTTGAGTGGATGGGATGGAACAGCGCTTACAATGGT10910.11483557776715848No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10580.1113620909969328No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10550.11104631947236683No Hit
CCTTGGGAGAATCCCCTGGATCACAGCTCCTCACCATGGACTGGACCTGG10550.11104631947236683No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10520.11073054794780085No Hit
CTATTATTGTGCGATGAGTTCTGAGGTGACGATTTTTCACGTGGGCCGTG10390.10936220467468165No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10240.10778334705185179No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10140.10673077530329855No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9920.10441511745648144No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9550.10052060198683443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)