FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804061
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36440.45319944631066544No Hit
ATAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAGCCTCAGGGAGA35880.4462348005934873No Hit
GTCTATTATTGTGTGAGCGGATTACAGTTATGGGGCGCGTCAGACAGTGG30110.3744740759718479No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28820.3584305170876339No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22730.28268999491332125No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22700.28231688889275813No Hit
ACTATACCCCGTCCCTCCAGAGTCGAGTCACCATATCCGTTGACACGTCC21800.2711237082758646No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21320.2651540119468548No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20320.25271714459475086No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20260.2519709325536247No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19880.24724492295982517No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19230.23916095918095764No Hit
CAATAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAGCCTCAGGGA17590.2187644967235073No Hit
CTATTATTGTGTGAGCGGATTACAGTTATGGGGCGCGTCAGACAGTGGTT17550.21826702202942314No Hit
GGGTATAGTGGGTGCTCCCACCAAAATAAATATTCCCAAGCCACTCCAGC17420.21665022927364963No Hit
GATTACAGTTATGGGGCGCGTCAGACAGTGGTTACTTCACTTTTGATTAC17150.2132922750885816No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17110.21279480039449744No Hit
ACTGTAATCCGCTCACACAATAATAGACAGCCGTGTCTGCGGCGGTCACA17050.2120485883533712No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCATGTACACTTTTGGCCAGG16850.20956121488295043No Hit
CTATACCCCGTCCCTCCAGAGTCGAGTCACCATATCCGTTGACACGTCCA16700.20769568478013484No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16350.2033427812068985No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16340.20321841253337744No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16140.20073103906295664No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15830.19687561018380448No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15140.18829417171085278No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14330.17822030915564863No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT14290.17772283446156448No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13060.1624254876184767No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12950.16105743220974528No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12920.16068432618918216No Hit
ATCTCTGGTGACTCCAGCAGCAGTAGCAGTTACTTCTGGGGCTGGATCCG12830.1595650081274928No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12420.15446589251313023No Hit
GTTATGGGGCGCGTCAGACAGTGGTTACTTCACTTTTGATTACTGGGGCC12390.1540927864925671No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12260.1524759937367936No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12140.15098356965454113No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12090.15036172628693595No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT11890.14787435281651518No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11760.14625756006074167No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT11570.14389455526384193No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG11520.14327271189623672No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11390.14165591914046324No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11330.14090970709933698No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11220.13954165169060556No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11200.1392929143435635No Hit
GTCCTGTCCCAGCCGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC10880.13531311679089025No Hit
CCCCATAACTGTAATCCGCTCACACAATAATAGACAGCCGTGTCTGCGGC10650.13245263729990633No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10630.13220389995286427No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10490.13046273852356974No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10170.1264829409708965No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA10150.1262342036238544No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10130.12598546627681234No Hit
GTCCCTCACCTGCACTATCTCTGGTGACTCCAGCAGCAGTAGCAGTTACT10020.1246174108680809No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10000.12436867352103884No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9900.12312498678582844No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGAGGCCCTACT9830.12225440607118118No Hit
GTATGGTAGCTCACCCATGTACACTTTTGGCCAGGGGACCAAGTTGGAGA9820.12213003739766012No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT9700.12063761331540766No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC9620.11964266392723935No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC9610.11951829525371832No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC9440.11740402780386065No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT9350.11628470974217131No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9260.11516539168048194No Hit
CACCTGCACTATCTCTGGTGACTCCAGCAGCAGTAGCAGTTACTTCTGGG9220.11466791698639779No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9210.11454354831287676No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9150.11379733627175054No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC9040.11242928086301911No Hit
ATTATTGTGTGAGCGGATTACAGTTATGGGGCGCGTCAGACAGTGGTTAC9010.11205617484245599No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT8950.11130996280132976No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG8770.10907132667795105No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA8650.10757890259569859No Hit
CTCCAGAGTCGAGTCACCATATCCGTTGACACGTCCAGAAACAAGGTCTC8650.10757890259569859No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8600.10695705922809338No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC8550.10633521586048819No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC8470.1053402664723199No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC8460.10521589779879884No Hit
CCCTGAGGCTGAGGTCTGTGACCGCCGCAGACACGGCTGTCTATTATTGT8410.10459405443119364No Hit
CATCTATGGTGCATCCAGTAGGGCCTCTGGCATCCCAGACAGGTTCAGTG8410.10459405443119364No Hit
GCACTATCTCTGGTGACTCCAGCAGCAGTAGCAGTTACTTCTGGGGCTGG8270.10285289300189912No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8230.10235541830781496No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8230.10235541830781496No Hit
GGGCTGGAGTGGCTTGGGAATATTTATTTTGGTGGGAGCACCCACTATAC8170.10160920626668872No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8100.10073862555204147No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8090.10061425687852041No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8060.10024115085795729No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8050.10011678218443626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)