FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011000
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCAGTGTCCTCAGTCTTTAGGCTGGTGATCTGAAGATATG15180.15014836795252226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14800.14638971315529178No Hit
GATTTATAGTACAAGCAAGAAACACTCCTGGACCCCTGCCCGGTTCTCAG11310.11186943620178041No Hit
GTCTATTACTGTGTGACGGGCAGCTCGCCCAACTGGTACTTCGATATATG10950.10830860534124628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17200.070.7781751
TCAACGC17900.065.968794
CAACGCA18700.063.143475
ATCAACG19250.061.345443
TATCAAC20600.057.6747742
AACGCAG20700.057.0426566
GGTATCA7250.055.640511
CGCAGAG23450.050.3507278
ACGCAGA26800.044.056897
GCAGAGT29250.040.3666539
ATATCGC850.002836821133.8818636
GTACATG23000.033.824941
ACATGGG22950.032.943963
TACATGG23950.032.1697352
CAGCGCT5350.029.6055859
TACAGCG1306.7512423E-427.6919085
GTACACG5950.026.6214645
CCAATAC5050.025.6655373
TACACCG1450.001277042724.8272275
CATGGGG15500.024.6202184
Produced by FastQC (version 0.11.5)