FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011000
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCAGTGTCCTCAGTCTTTAGGCTGGTGATCTGAAGATATG14550.14391691394658754No Hit
GATTTATAGTACAAGCAAGAAACACTCCTGGACCCCTGCCCGGTTCTCAG12090.11958456973293768No Hit
GTCTATTACTGTGTGACGGGCAGCTCGCCCAACTGGTACTTCGATATATG10800.10682492581602374No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10730.10613254203758656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11850.051.6647454
GTATCAA12000.051.634491
ATCAACG12700.048.776423
CAACGCA13250.046.203545
AACGCAG14500.042.2016756
TATCAAC14950.040.9597662
CGCAGAG15450.039.602838
GGTATCA6200.036.0240671
ACGCAGA18850.032.461227
GTACATG23600.030.528871
TATACCG950.00488237230.3255465
GCAGAGT20850.030.0364769
GTACACG4900.029.3972115
TACATGG25750.027.9770852
GTATAGG3750.026.8979681
ATACCGT1358.4222376E-426.663376
ACCGACC1358.4271067E-426.660738
ACATGGG24250.026.138833
TACACGG5950.024.1986876
AGTACCC2151.963484E-523.4494045
Produced by FastQC (version 0.11.5)