FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548566
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15120.275627727566054No Hit
ATATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGCCGAGT15020.2738047928599293No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12260.22349179497088775No Hit
ATAATATATAAGGCCAATCCACTCCAGTCGCTTCCCTGGGGGCTGCCGGA10030.182840351024307No Hit
GCCTTATATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGC9840.17937677508267008No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA9290.16935063419898427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.16898604725775931No Hit
GTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT9170.16716311255163463No Hit
GTGCAAAGAGGTTGCACCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT8580.1564077977854989No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8490.1547671565499867No Hit
ATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGCCGAGTCA8290.15112128713773731No Hit
GTTATAGTCCCCGTCCGATCACCTTCGGCCAAGGGACACGACTGGATATT8260.1505744067258999No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7290.13289194007649036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7260.13234505966465293No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7170.1307044184291407No Hit
ATATAAGGCCAATCCACTCCAGTCGCTTCCCTGGGGGCTGCCGGATCCAA7020.12797001636995367No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTACGAA7010.12778772289934118No Hit
GTATTACGAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT6950.12669396207566636No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6950.12669396207566636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6930.12632937513444145No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6920.12614708166382899No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6860.12505332084015416No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6840.1246887338989292No Hit
GAGTTATAGTCCCCGTCCGATCACCTTCGGCCAAGGGACACGACTGGATA6800.12395956001647933No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6740.1228657991928045No Hit
CTCTTTGCACAGTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGA6730.12268350572219204No Hit
TCTTTGCACAGTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGAC6580.11994910366300499No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6320.1152094734270808No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6230.11356883219156856No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5910.10773544113196953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5870.10700626724951966No Hit
GACTATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA5870.10700626724951966No Hit
CTATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT5800.10573021295523237No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5730.10445415866094508No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5690.1037249847784952No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA5690.1037249847784952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.083.683361
ATCAACG14200.073.0223163
TCAACGC14350.072.259024
CAACGCA15300.067.766175
AACGCAG15700.066.0396356
TATCAAC15700.065.587012
CGCAGAG17050.060.8051458
GCAGAGT19600.052.8942769
ACGCAGA19850.052.2281047
TAGTACG450.00989951148.005414
GTACATG13450.034.824861
GAATACG1905.798938E-934.102899
GTGCTAG2353.6379788E-1133.7305261
ACATGGG13250.033.6943633
TACATGG13450.033.193332
GTGTAGA3700.033.1089551
GATACTA900.003749750332.0036052
CATGGGG8150.030.0401954
GGAGTTA11050.029.3192277
GAGTTAC11100.029.1871558
Produced by FastQC (version 0.11.5)