FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548566
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGCCGAGT15340.2796381839195284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15260.2781798361546286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11900.21692923002883882No Hit
ATAATATATAAGGCCAATCCACTCCAGTCGCTTCCCTGGGGGCTGCCGGA11880.2165646430876139No Hit
GTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA9990.18211117714185715No Hit
GCCTTATATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGC9570.17445485137613342No Hit
GTGCAAAGAGGTTGCACCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT9150.16679852561040967No Hit
GTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGACAGATTTCACT9080.16552247131612238No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8820.1607828410801982No Hit
ATATAAGGCCAATCCACTCCAGTCGCTTCCCTGGGGGCTGCCGGATCCAA8690.15841302596223608No Hit
ATATTATAGTGGAACCACAAATTACAACCCCTCCCTCAACAGCCGAGTCA8310.15148587407896225No Hit
GTTATAGTCCCCGTCCGATCACCTTCGGCCAAGGGACACGACTGGATATT8240.15020981978467496No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7880.14364725484262603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7810.14237120054833877No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7600.1385430376654769No Hit
GTATTACGAAGTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCT7170.1307044184291407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7160.13052212495852825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7140.1301575380173033No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTACGAA7120.12979295107607836No Hit
GAGTTATAGTCCCCGTCCGATCACCTTCGGCCAAGGGACACGACTGGATA7100.12942836413485342No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7020.12797001636995367No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC6910.1259647881932165No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6630.12086057101606736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6460.11776158201565537No Hit
CTCTTTGCACAGTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGA6410.11685011466259301No Hit
CTATAATATATAAGGCCAATCCACTCCAGTCGCTTCCCTGGGGGCTGCCG6260.11411571260340597No Hit
TCTTTGCACAGTGGGGTCCCATCGAGATTCAGTGGCAGTGGATCTGGGAC6240.11375112566218103No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5980.10901149542625682No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5930.10810002807319448No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5890.1073708541907446No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT5810.10591250642584484No Hit
CTATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT5690.1037249847784952No Hit
GACTATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA5630.10263122395482038No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5520.10062599577808323No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5500.10026140883685829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCG300.001989158371.999829
TCAACGC6900.053.2366754
ATCAACG7600.048.3332943
CAACGCA7700.047.705595
GTATCAA8350.045.7296681
GAATACG2250.044.7998859
TATCAAC8250.044.5333442
AACGCAG8300.043.3733866
CGCAGAG8750.041.1427548
GTGCTAG3150.038.8818631
TACATGG13250.038.602152
TACACCG750.001531362638.4139145
ACATGGG13250.036.964333
GTACATG13950.036.1520161
CTAGAGA3850.033.6745344
GCCTTAT6800.032.844411
GCTAGAG3750.032.651833
GCAGAGT11350.031.7179839
CCTTATA7300.031.578842
CCTATAC1601.9735326E-631.5114143
Produced by FastQC (version 0.11.5)