FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801096
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGA41430.51716648191977No Hit
GTATAATAGTTATTCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA27020.3372879155556887No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG26360.32904920259244835No Hit
GTCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTATATTA23060.28785563777624656No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT22830.28498457113754155No Hit
GTATATTACTGTGCGAGGTCCCCCCCCGATTACTATGATAATAGTGGTTC19650.24528895413283802No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18570.2318074238293538No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG17740.22144661813315755No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCA17700.22094730219599149No Hit
ATATACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGACTG16970.2118347863427105No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16970.2118347863427105No Hit
CATGTAGACTGTGCTCGTGGACGTGTCCCTAGTCATGGTGACTCTGCCCT16390.20459470525380227No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCCTAGTCATGGTGACTCTGCCC15660.1954821894005213No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14820.18499655472003354No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13430.1676453259035122No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13400.16727083895063763No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC13320.16627220707630547No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13200.1647742592648072No Hit
GTGCATATAGTAGGTGGTGAAGATGTATCCAGATGCCTTGCAGGACACCT12570.15691003325444142No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11930.1489209782597841No Hit
TAGTTATTCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAAC11710.14617474060537064No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11350.1416808971708759No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG11080.13831051459500485No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG10930.13643807983063203No Hit
CTATTATCATAGTAATCGGGGGGGGACCTCGCACAGTAATATACGGCCGT10830.13518978998771683No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10690.13344218420763554No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC10620.13256838131759488No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10610.13244355233330338No Hit
GAATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG9930.12395518140147997No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9820.12258206257427325No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9720.12133377273135804No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9670.12070962780990042No Hit
CAGTAATATACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTA9550.1192116799984022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.11609095539111416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9250.11546681046965657No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9210.11496749453249047No Hit
CAGTATAATAGTTATTCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGA9070.11321988875240921No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8940.11159711195661944No Hit
GCCGTATATTACTGTGCGAGGTCCCCCCCCGATTACTATGATAATAGTGG8830.1102239931294127No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGAGTCCCATCAAGGTTCAGCGGC8750.10922536125508055No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT8710.10872604531791447No Hit
ATATTACTGTGCGAGGTCCCCCCCCGATTACTATGATAATAGTGGTTCCC8690.10847638734933142No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8570.10697843953783318No Hit
GTAAGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAG8430.10523083375775187No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8240.102859083056213No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8150.1017356221975893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)