FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780697
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.19239218288273172No Hit
CGTATATACTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCGG12490.15998524395508118No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12080.154733526579454No Hit
ATATAGGGCCGTGTCGTCGGCGCTCAGGCTGCTCATGTGCAAGTAGAGCG9100.1165625076053834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8480.10862088620809354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.072.454171
ATCAACG21350.064.141533
TCAACGC21600.063.399154
CAACGCA22050.062.1052975
AACGCAG23400.058.5185436
TATCAAC23450.058.397522
GGTATCA7250.053.683321
CGCAGAG25800.053.5786828
ACGCAGA25750.053.4373447
GCAGAGT30150.045.6096239
CAGAGTA29600.028.2147879
TGAACCG1050.00793183227.4570775
AGCGGTC1658.824873E-526.1804928
GTATTAA5350.025.5966681
GGGCCGT6200.025.57336
GTACATG23400.025.5650021
TACATGG24150.025.0695062
ACATGGG23700.024.6331673
TAGTACG2101.6269281E-524.0249424
CCCTATA5150.023.791692
Produced by FastQC (version 0.11.5)