FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632103
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19440.30754481469001094No Hit
CCTATGGGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACG17640.2790684429594544No Hit
ATGTATTACTGTGCGAAAACTTGTGACAGTAGTGGCCAGGAATGCTTTGA16760.26514666122451563No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA16240.25692015383568817No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13870.21942626439045537No Hit
GTAATACATGGCGCTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGA12950.20487167439483756No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12420.19648696494084036No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12170.1925319133115964No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10860.17180744277435797No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10420.1648465519068886No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTCCTGGTCATCTATTTTGAC10380.16421374364620955No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10160.16073329821247487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.1583602672349285No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC9610.15203218462813814No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG9590.15171578049779863No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9580.15155757843262885No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9440.14934274952025223No Hit
GTCCTGGTCATCTATTTTGACAGCGACCGGCCCTCAGGGATCCCTGAGCG9350.1479189309337244No Hit
GGCTCTGTGACCTCCTATGGGCTGACTCAGCCACCCTCAGTGTCAGTGGC9150.14475488963032923No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8980.14206545452244335No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8950.14159084832693405No Hit
CAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCTGATGGCCAGGGT8820.1395342214797272No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA8810.13937601941455743No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA8690.13747759463252032No Hit
CTATTATTGTCAGGTGTGGGATGCTGGTAGTGATCATTGGGTGTTCGGCG8680.13731939256735057No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8580.135737371915653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8300.13130771409089975No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8210.1298838955043719No Hit
CCCAATGATCACTACCAGCATCCCACACCTGACAATAATAGTCGGCCTCA8160.1290928851785231No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8000.12656165213580697No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA7830.12387221702792109No Hit
CTACCAGCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGTCGTC7760.12276480257173278No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7700.12181559018071422No Hit
CCCCTGTCCTGGTCATCTATTTTGACAGCGACCGGCCCTCAGGGATCCCT7700.12181559018071422No Hit
GGACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTTACTT7670.12134098398520493No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7630.12070817572452591No Hit
CCCCAGGACAGACGGCCAGGTTGACCTGTGGGGGAAACAACATTGGAAGT7620.12054997365935614No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7530.11912615507282831No Hit
GACCAGGACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTT7480.11833514474697952No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7420.11738593235596098No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7330.11596211376943315No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGGGCTGACTCAGCCAC7200.11390548692222628No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7100.1123234662705287No Hit
TCCCACACCTGACAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCT6900.10915942496713352No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT6870.10868481877162424No Hit
ATCCTAGTGACTCCGATACCAAATACAGCCCGTCCTTCCAAGGCCAAGTC6830.1080520105109452No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC6820.10789380844577545No Hit
ATTATTGTCAGGTGTGGGATGCTGGTAGTGATCATTGGGTGTTCGGCGGA6810.1077356063806057No Hit
GTCTGGAGCAGAGGCGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTA6630.10488796920755004No Hit
GACAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCTGATGGCCAGG6600.10441336301204075No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6530.10330594855585244No Hit
GCACAGGCTCTGTGACCTCCTATGGGCTGACTCAGCCACCCTCAGTGTCA6410.10140752377381534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13350.072.8603441
ATCAACG15800.061.0628553
TCAACGC15950.060.4885944
CAACGCA16600.058.1200685
TATCAAC16750.058.0294342
GGTATCA5500.057.6406251
AACGCAG16950.057.3447236
CGCAGAG18550.052.3943988
GCAGAGT20600.047.1803979
ACGCAGA21250.046.0760277
CAGCGCT2151.2732926E-1136.834039
TATTGAG8450.035.7867285
ACATGGG14800.034.540293
TACACCG850.002835919433.882115
TTTCGCG850.002837019533.879439
GTACATG16000.033.7738041
GAATACG1957.477865E-933.2279059
TTATTGA9650.032.0826724
TACACGG4050.030.2220086
TGGGAGT6050.029.7518546
Produced by FastQC (version 0.11.5)