FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632103
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20300.3211501922946102No Hit
GTAATACATGGCGCTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGA18770.29694527632363715No Hit
CCTATGGGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACG18360.29045899165167705No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA15690.24821904025135144No Hit
ATGTATTACTGTGCGAAAACTTGTGACAGTAGTGGCCAGGAATGCTTTGA15500.24521320101312602No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14460.22876018623547112No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14360.22717816558377352No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11670.18462181005310843No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10590.16753598701477448No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTCCTGGTCATCTATTTTGAC10240.16199891473383293No Hit
GTCCTGGTCATCTATTTTGACAGCGACCGGCCCTCAGGGATCCCTGAGCG10000.15820206516975874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9900.15662004451806114No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9570.1513993763674591No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC9370.14823533506406392No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG9350.1479189309337244No Hit
GGCTCTGTGACCTCCTATGGGCTGACTCAGCCACCCTCAGTGTCAGTGGC9130.14443848549998972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9060.1433310710438014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8950.14159084832693405No Hit
CAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCTGATGGCCAGGGT8930.14127444419659455No Hit
CTATTATTGTCAGGTGTGGGATGCTGGTAGTGATCATTGGGTGTTCGGCG8770.1387432111538784No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8670.1371611905021808No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8530.1349463615898042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8350.13209872441674853No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA8180.12940928930886264No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8090.1279854707223348No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8030.12703625833131627No Hit
CCCCTGTCCTGGTCATCTATTTTGACAGCGACCGGCCCTCAGGGATCCCT7810.12355581289758155No Hit
CCCAATGATCACTACCAGCATCCCACACCTGACAATAATAGTCGGCCTCA7760.12276480257173278No Hit
GGACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTTACTT7750.12260660050656301No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA7650.12102457985486542No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG7630.12070817572452591No Hit
CTACCAGCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGTCGTC7580.11991716539867711No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7580.11991716539867711No Hit
CCCCAGGACAGACGGCCAGGTTGACCTGTGGGGGAAACAACATTGGAAGT7510.1188097509424888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA7500.11865154887731905No Hit
TCCCACACCTGACAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCT7420.11738593235596098No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7420.11738593235596098No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7310.11564570963909362No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGGGCTGACTCAGCCAC7310.11564570963909362No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7250.11469649724807507No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7200.11390548692222628No Hit
GACCAGGACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTT7020.11105784974917063No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT6960.11010863735815207No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG6610.10457156507721052No Hit
ATTATTGTCAGGTGTGGGATGCTGGTAGTGATCATTGGGTGTTCGGCGGA6580.10409695888170124No Hit
GACAATAATAGTCGGCCTCATCCCCGTCGTCGACCCTGCTGATGGCCAGG6460.10219853409966413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)