FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014588
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGACAGGTCTAAGGACCAGTTCTCCCTGAAGCTGAACTCTGTGAC30580.3014031311231751No Hit
GTCTATGGATATGGTGATTCGACTCTTGAGGGACGGGTTGTAGAAGGTGG24850.2449270048532015No Hit
TCTATAAGGTGTCTACTTTAGAAAGCGGGGTCCCATCAAGGTTCAGCGGC19990.1970257878074647No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18560.18293139678371909No Hit
GTCTCTGGTGGCTCCATGATCAATCCTACTTACTACTGGGGCTGGATCCG18130.17869322325909628No Hit
GTATAGTGGGGGTTGGTCGTTCGGCCAAGGGACCACGGTGGAAATCAAAC17200.1695269409849121No Hit
CCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG13180.12990494663843846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12590.12408977831395601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.064.051811
TCAACGC16850.063.3602334
ATCAACG17750.060.5540053
CAACGCA18750.056.9453665
TATCAAC19150.056.8832782
AACGCAG19750.054.711426
ACGCAGA20900.051.7035377
CGCAGAG21000.051.114288
GAATACG1601.0750227E-940.5226489
GCAGAGT27250.039.390829
CGTATAC800.002087496736.068243
TCTATGG14950.029.9176122
GTACATG31850.029.4551161
GTCTATG15600.028.6809791
TACATGG32000.027.954272
CATAGAC17800.027.1524963
CTATGGA15900.026.3139363
TATGGAT16500.025.7942564
ACATGGG36600.025.0309683
GACAGGT17200.024.7114877
Produced by FastQC (version 0.11.5)