FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592849
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21410.36113749032215625No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18340.30935364654406095No Hit
CTCTAGTACAGTAATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATT14100.2378345919449978No Hit
ATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATTTGCAGATAGGCGA14070.23732856089830634No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13490.2275452939956043No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13250.22349704562207243No Hit
CTCCTGTACAGCTTCTGGATTCGCCTTTGGTGATTATGCTATGAGCTGGG12360.20848479123689168No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12010.2025810956921577No Hit
GTACTAGAGCATATGATACTAGTGGTTATTACTCTTACTACTGGGGCCAG11200.1889182574314876No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10860.18318323890231747No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10070.16985775467277503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.16058051881676447No Hit
GCCTTGTATTACTGTACTAGAGCATATGATACTAGTGGTTATTACTCTTA8820.1487731277272965No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8790.148267096680605No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8390.1415200160580519No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7930.13376087334211578No Hit
CAGTAATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATTTGCAGATA7710.13004997899971155No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGCTGGAGTCTGGGGGAGGCT7690.1297126249685839No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7370.1243149604705414No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7130.12026671209700952No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7110.11992935806588187No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCGCCTTTGGTGATTATGCT7030.11857994194137125No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6790.11453169356783936No Hit
GTATTACTGTACTAGAGCATATGATACTAGTGGTTATTACTCTTACTACT6420.10829064399197773No Hit
GTAGTAAGAGTAATAACCACTAGTATCATATGCTCTAGTACAGTAATACA6270.1057604887585203No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6120.10323033352506288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)