FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592849
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23690.39959584987070906No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17140.2891124046764016No Hit
ATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATTTGCAGATAGGCGA13740.23176221938469999No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13530.22822000205785958No Hit
CTCTAGTACAGTAATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATT13410.22619587787109366No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13030.21978615127966816No Hit
CTCCTGTACAGCTTCTGGATTCGCCTTTGGTGATTATGCTATGAGCTGGG11750.1981954932874982No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC11420.19262915177389184No Hit
GTACTAGAGCATATGATACTAGTGGTTATTACTCTTACTACTGGGGCCAG11140.18790619533810465No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11090.1870628102602855No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8900.15012254385180712No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8810.14860445071173267No Hit
GCCTTGTATTACTGTACTAGAGCATATGATACTAGTGGTTATTACTCTTA8700.14674900354053055No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8580.1447248793537646No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGCTGCTGGAGTCTGGGGGAGGCT7800.13156807213978602No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7630.12870056287520093No Hit
CAGTAATACAAGGCTGTGTCCTCGGTTTCCAGGCTGTTGATTTGCAGATA7290.12296554434603078No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7170.12094142015926485No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT6890.11621846372347766No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6880.11604978670791383No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCGCCTTTGGTGATTATGCT6850.11554375566122234No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA6760.11402566252114787No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC6720.11335095445889257No Hit
GTAGTAAGAGTAATAACCACTAGTATCATATGCTCTAGTACAGTAATACA6680.11267624639663726No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA6560.11065212220987132No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6530.11014609116317983No Hit
GTATTACTGTACTAGAGCATATGATACTAGTGGTTATTACTCTTACTACT6420.10829064399197773No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC6060.1022182714316799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7650.050.843761
TCAACGC7800.049.861794
ATCAACG8000.048.6152423
CAACGCA8200.048.307835
TATTACG450.00988874848.0191042
AACGCAG8450.046.862786
TATCAAC8550.045.4917872
GGTATCA4100.042.1631161
ACGCAGA9650.041.0352827
CGCAGAG9950.039.798048
GTACATG13950.038.725091
TACATGG14000.037.55782
CATGGGA5900.035.400934
CCCCGTT3700.035.0261847
ACATGGG14850.034.920043
GTCATAG9300.034.8525771
CCCGTTG3850.033.661538
ATAGGTG9150.033.0595474
GCACACA8850.032.5553252
GCAGAGT12050.032.2647869
Produced by FastQC (version 0.11.5)