FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866463
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGAGA49480.5710572753827919No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGGGCTGGATCCG34430.3973626109828117No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA33510.3867447311656701No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT32950.3802816738856708No Hit
ATGTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACCACA28310.32673062785139123No Hit
ATATAACACTTATTCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC26180.30214792783996547No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22400.25852229119997044No Hit
GTGTTATATTGCTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCT22330.25771440903997056No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG21630.24963558743997144No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA21390.24686570574854322No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC20780.23982558978282972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19680.2271302986971169No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19000.21928230057140352No Hit
CAGTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGA18480.21328089023997565No Hit
GAGTATTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC18190.20993394986283312No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACCTCC16850.19446877708569207No Hit
GTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACCACATG16180.18673619069712152No Hit
CCCCTAAAGGGGCCTCTCGCACAGTAATACATGGCCGTGTCCGCGGCGGT15920.18373548553140756No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15520.1791190160456938No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT15060.173810076137123No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14870.17161725313140894No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT14610.168616547965695No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGG14220.1641154902171241No Hit
ATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGAGAACT14090.16261513763426713No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGAC14080.16249972589712428No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13750.15869113857141043No Hit
CATCAAGGGCATGTGGTATAACCGGAAGACCCCTAAAGGGGCCTCTCGCA13710.15822949162283906No Hit
TATCAATAGACACCTCCAAGAACCAGTTCTCCCTGAGACTGAGCTCTGTG13410.15476713950855375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.15084314044569705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12870.14853490570284017No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCATCCGCCT12730.14691914138284035No Hit
GGTATAACCGGAAGACCCCTAAAGGGGCCTCTCGCACAGTAATACATGGC12420.14334137753141218No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT12390.14299514231998364No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12260.14149478973712667No Hit
GTATTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT12160.14034067236569825No Hit
ATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCG12150.1402252606285554No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12120.13987902541712688No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCACTGTCCCGTGG12020.13872490804569843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11830.1365320850399844No Hit
GTCACATCCTGGCTGGAATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11730.13537796766855598No Hit
GCCATGTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACC11670.1346854972456989No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAT11590.13376220334855615No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA11430.13191561555427064No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTG11340.13087690991998505No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11190.1291457338628424No Hit
GGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTC11180.12903032212569954No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11070.12776079301712825No Hit
GTATGCTGGTTACAGCGTAGGTGGTGGTGCCCTGGCTGGGCTCCTGCTGG10970.1266066756456998No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT10970.1266066756456998No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10740.12395220569141441No Hit
CTTTAGGGGTCTTCCGGTTATACCACATGCCCTTGATGATTCTTTTGATA10690.1233751470057002No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10690.1233751470057002No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG10570.12199020615998606No Hit
GTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC10330.11922032446855779No Hit
GTCTCAGGGAGAACTGGTTCTTGGAGGTGTCTATTGATATGGTGACTCGA9700.11194938502855864No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCGGACACGGCCATGTATTACTGT9680.11171856155427295No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9670.1116031498171301No Hit
GTCTATTGATATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGC9660.11148773807998726No Hit
TCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGC9650.1113723263428444No Hit
GTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG9550.11021820897141596No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCA9470.10929491507427323No Hit
GTAGTAGGTGCTCCCACTATAATAGATACTCCCAATCCACTCCAGCCCCT9410.10860244465141616No Hit
CTATTGATATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGCTC9390.10837162117713046No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9310.10744832727998772No Hit
TCAATAGACACCTCCAAGAACCAGTTCTCCCTGAGACTGAGCTCTGTGAC9180.10594797469713074No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9180.10594797469713074No Hit
GGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGTCGAGGCTCAGC8950.10329350474284534No Hit
GTCACGGACTTGCCGTCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8890.10260103431998828No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACT8800.10156232868570268No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTA8760.1011006817371313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8710.10052362305141707No Hit
CACTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGG8670.10006197610284571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18050.071.8636861
ATCAACG20050.063.9727063
TCAACGC20450.063.0737724
CAACGCA21350.060.4114385
TATCAAC21200.060.1625862
AACGCAG22550.057.1933336
CGCAGAG24500.052.310878
ACGCAGA24700.052.1998987
CCGTCGC450.00990759647.997859
GCAGAGT28450.045.045439
GTGCTAG2400.039.0342861
GAATACG2300.037.563539
GTACATG23150.035.4868971
ACATGGG23650.034.125263
TCGCTGC3600.032.0281371
CTTGCGC1153.276884E-431.3300633
CATGGGA11450.030.8375364
AACCGTG950.0048797230.328437
ACCGACC3850.029.9224628
GCACCGA4100.029.874736
Produced by FastQC (version 0.11.5)