FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866463
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGAGA47120.5438201054170807No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGGGCTGGATCCG35300.40740343211423913No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA32140.3709333231771005No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT32050.3698946175428149No Hit
ATGTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACCACA26650.30757227948567917No Hit
ATATAACACTTATTCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC25170.29049134238853824No Hit
GTGTTATATTGCTGGCAGTAATAACTTGCAAAATCATCAGGCTGCAGGCT22650.2614075846285415No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21400.24698111748568605No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG20990.24224923626282946No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA20920.24144135410282952No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC19020.2195131240456892No Hit
GAGTATTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC17750.20485583342854802No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17660.20381712779426242No Hit
CAGTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGA17340.20012395220569143No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACCTCC16640.19204513060569234No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15840.18281219163426482No Hit
GTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACCACATG15660.18073478036569363No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT15610.1801577216799794No Hit
CCCCTAAAGGGGCCTCTCGCACAGTAATACATGGCCGTGTCCGCGGCGGT15200.1754258404571228No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14930.17230972355426602No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14120.16296137284569567No Hit
ATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTCTCAGGGAGAACT13810.1593836089942675No Hit
CATCAAGGGCATGTGGTATAACCGGAAGACCCCTAAAGGGGCCTCTCGCA13760.15880655030855326No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGAC13760.15880655030855326No Hit
GTCATGGAACCATGGGGAGACCTTCACCTGCACTGCTGCCCACCCCGAGT13540.15626749209141072No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGG13360.15419008082283953No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT12940.14934278786284008No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12750.14714996485712603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12500.14426467142855495No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT12260.14149478973712667No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTG12160.14034067236569825No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12160.14034067236569825No Hit
ATCTTGGGGGGTGCTGTCGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCG12100.1396482019428412No Hit
TATCAATAGACACCTCCAAGAACCAGTTCTCCCTGAGACTGAGCTCTGTG12080.13941737846855548No Hit
GTATTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT12070.13930196673141265No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11370.13122314513141356No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11160.12879949865141385No Hit
GGTATAACCGGAAGACCCCTAAAGGGGCCTCTCGCACAGTAATACATGGC11060.12764538127998543No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11020.12718373433141403No Hit
GGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTC11010.1270683225942712No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT10750.12406761742855725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10710.12360597047998588No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCACTGTCCCGTGG10650.12291350005712881No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10400.1200282066285577No Hit
GTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC10360.11956655967998633No Hit
CTTTAGGGGTCTTCCGGTTATACCACATGCCCTTGATGATTCTTTTGATA10310.11898950099427212No Hit
GTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG10300.11887408925712926No Hit
GCCATGTATTACTGTGCGAGAGGCCCCTTTAGGGGTCTTCCGGTTATACC10170.1173737366742723No Hit
GTCTATTGATATGGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGC10050.11598879582855819No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCATCCGCCT9920.11448844324570119No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9910.11437303150855836No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAT9860.11379597282284414No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG9850.1136805610857013No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA9770.11275726718855854No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9620.11102609113141587No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCGGACACGGCCATGTATTACTGT9620.11102609113141587No Hit
GTCTCAGGGAGAACTGGTTCTTGGAGGTGTCTATTGATATGGTGACTCGA9620.11102609113141587No Hit
TCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9540.11010279723427313No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACT9500.10964115028570176No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9240.10664044511998783No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9060.10456303385141662No Hit
GTCACATCCTGGCTGGAATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9000.10387056342855956No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCA8990.10375515169141672No Hit
TCAATAGACACCTCCAAGAACCAGTTCTCCCTGAGACTGAGCTCTGTGAC8980.10363973995427386No Hit
GTAGTAGGTGCTCCCACTATAATAGATACTCCCAATCCACTCCAGCCCCT8820.10179315215998837No Hit
GCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGG8780.10133150521141698No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAAGTTA8680.10017738783998854No Hit
TCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGC8670.10006197610284571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.065.359171
ATCAACG14400.063.5960433
TCAACGC14750.062.575864
TATCAAC14800.062.364452
CAACGCA16950.054.4633755
AACGCAG17900.051.897686
ACGCAGA18500.050.603777
CGCAGAG18800.049.413228
GCAGAGT20350.045.6495559
CATTAGC5800.034.7649238
ACATTAG6550.031.8836467
GTACATG26200.031.3919771
ACGGTGT950.00488534130.321298
ACATGGG26750.030.191393
ATTAGCA6700.030.095019
TTTCGAG1050.00791402427.4700582
GTGCTAG3750.026.9346831
GCTTGGG2705.858965E-926.7209131
AATCCGC1907.538407E-626.5311286
CCGCTAC1907.538407E-626.5311289
Produced by FastQC (version 0.11.5)