FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732740
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACGGCCGTGTCCTCGGCTCTCAGGGTGCTCATTTGCAGAAACA53770.7338210006277807No Hit
GTCTACTACTGTGCGAAGAGGAAAGGTTTCGATATAGACGAGTTTGACAA31770.4335780768075989No Hit
CTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGTCGGGGGGACGCT26300.3589267680213991No Hit
TTAGTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCT25690.35060185058820315No Hit
GTATACTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTC23020.3141632775609357No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20410.2785435488713595No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18450.251794633840107No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18010.24578977536370336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16010.21849496410732322No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15220.20771351366105306No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCCGGG14350.19584027076452767No Hit
CTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTCAACGA13560.1850588203182575No Hit
GGTAATTAGTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGC13520.18451292409312992No Hit
CACTAATACCTGAGACCCACTCAAGCCCCTTCCCTGGAGTCTGGCGGACC13470.1838305538117204No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC12890.17591505854737016No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12800.17468679204083304No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12680.17304910336545024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.16622540055135518No Hit
GTATTAGTGATAGTGGTGGTAGTTCATATTACGCAGACTTCGCGAAGGGC11880.16213117886289816No Hit
GTATTAGCACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT11800.16103938641264295No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.1557168982176488No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGCT11290.15407920954226603No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11050.1508038321915004No Hit
GTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCCTTGAGCTGGGTCCGC10580.14438955154625105No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10330.14097770013920355No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCCTTGAGCTGGG10270.14015885580151213No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGGGCTGGCTTTTT10160.13865764118241122No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10070.13742937467587413No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9970.1360646341130551No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9720.13265278270600758No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9630.1314245161994705No Hit
GACCTACACCTGCAACGTAGATCATAAGCCCAGCAACACCAAGGTGGACA9470.12924093129896005No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC9330.12733029451101344No Hit
TCCCTGGAGTCTGGCGGACCCAGCTCAAGGCATAGCTACTAAAGGTGAAT9000.12282665065371073No Hit
CAGTATACTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGAT8950.12214428037230123No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8890.12132543603460982No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8820.12037011764063651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8770.119687747359227No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8760.11955127330294511No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC8730.11914185113409942No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA8620.1176406365149985No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGGGCTGGCTTTTTCTTGT8610.1175041624587166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8580.11709474028987091No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT8520.11627589595217949No Hit
CCTTTAGTAGCTATGCCTTGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG8500.11600294783961568No Hit
CCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCC8490.11586647378333378No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8300.11327346671397766No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG8190.11177225209487676No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT8100.11054398558833967No Hit
GTCTGCATCTATAGGAGACGGAGTCACCATCACTTGTCGGGCGAGTCAAG8070.11013456341949394No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG8070.11013456341949394No Hit
CTTCAGAGACAATACCAAGAACACGGTGTTTCTGCAAATGAGCACCCTGA7950.10849687474411114No Hit
GTATCAACGCAGAGTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGG7950.10849687474411114No Hit
GTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGT7940.10836040068782923No Hit
AGTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGG7890.10767803040641975No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT7800.10644976389988263No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT7580.10344733466168081No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7520.10262849032398941No Hit
ATATAGACGAGTTTGACAACTGGGGCCAGGGAACCCCGGTCACCGTCTCC7510.1024920162677075No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC7470.10194612004257991No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT7380.10071785353604279No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA7370.1005813794797609No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT7360.100444905423479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.075.8006441
ATCAACG16350.065.66983
TCAACGC16450.064.832534
CAACGCA16550.064.4407965
TATCAAC16950.062.9200632
AACGCAG17350.061.4694636
GAATACG1900.056.8393829
CGCAGAG19450.055.1541758
GCAGAGT21900.048.983969
AGACGGC19050.041.9886
GTAGACG18550.041.954354
TAGACGG19050.041.6097265
GACGGCC19000.041.3230257
AGTAGAC19250.041.1774183
GTAGTAG19500.041.0190431
GTGCTAG2750.039.3057141
TAGTAGA20850.037.6719062
TACATGG16000.037.3813742
GTACATG16150.037.0341761
ACATGGG16600.036.030243
Produced by FastQC (version 0.11.5)