FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732740
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACGGCCGTGTCCTCGGCTCTCAGGGTGCTCATTTGCAGAAACA50110.683871496028605No Hit
GTCTACTACTGTGCGAAGAGGAAAGGTTTCGATATAGACGAGTTTGACAA31270.4267543739935038No Hit
CTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGTCGGGGGGACGCT24170.32985779403335425No Hit
GTATACTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTC23930.32658241668258864No Hit
TTAGTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCT23370.3189398695308022No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19530.2665338319185523No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT17000.23200589567923136No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16920.23091410322897618No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16820.22954936266615716No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCCGGG14070.19201899718863444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14010.19120015285094305No Hit
GGTAATTAGTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGC13860.18915304200671454No Hit
CTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGATTCAACGA12950.17673390288506155No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC12890.17591505854737016No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12150.1658159783825095No Hit
CACTAATACCTGAGACCCACTCAAGCCCCTTCCCTGGAGTCTGGCGGACC11990.1636323934819991No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGCT11830.16144880858148866No Hit
GTATTAGTGATAGTGGTGGTAGTTCATATTACGCAGACTTCGCGAAGGGC11290.15407920954226603No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11010.15025793596637277No Hit
GTATTAGCACCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT10910.14889319540355378No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10530.14370718126484155No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10450.14261538881458632No Hit
GTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCCTTGAGCTGGGTCCGC10310.14070475202663973No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10140.1383846930698474No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10140.1383846930698474No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCCTTGAGCTGGG9800.13374457515626278No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA9760.1331986789311352No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9350.12760324262357728No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGGGCTGGCTTTTT9300.12692087234216776No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9280.12664792422960397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9240.12610202800447634No Hit
GACCTACACCTGCAACGTAGATCATAAGCCCAGCAACACCAAGGTGGACA8940.12200780631601933No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT8780.1198242214155089No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8520.11627589595217949No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8460.11545705161448809No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC8420.11491115538936048No Hit
TCCCTGGAGTCTGGCGGACCCAGCTCAAGGCATAGCTACTAAAGGTGAAT8370.11422878510795097No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC8350.11395583699538717No Hit
CCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTTAGTAGCTATGCC8260.11272757048885007No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG8120.11081693370090347No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8100.11054398558833967No Hit
CCTTTAGTAGCTATGCCTTGAGCTGGGTCCGCCAGACTCCAGGGAAGGGG8050.10986161530693016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8020.10945219313808444No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT7890.10767803040641975No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT7760.10590386767475504No Hit
CAGTATACTAATTACCCGCTCACCTTCGGCCAAGGGACACGACTGGAGAT7760.10590386767475504No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGGGCTGGCTTTTTCTTGT7720.10535797144962743No Hit
GTCTGCATCTATAGGAGACGGAGTCACCATCACTTGTCGGGCGAGTCAAG7710.10522149739334552No Hit
GTATACTGTTGGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGT7630.10412970494309032No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7620.10399323088680842No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA7520.10262849032398941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7480.1020825940988618No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT7480.1020825940988618No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC7360.100444905423479No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)