FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631894
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.17059823324798148No Hit
GTATTACTGTCAGCATTATGCTAGCTCACCGTGGACGTTCGGCCAGGGGA6530.10334011717155092No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6440.10191582765463829No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.071.4539261
GTATCAA14300.069.530171
TCAACGC15500.063.2075044
CAACGCA15900.062.5235185
ATCAACG15700.062.4023133
AACGCAG16900.059.250166
TATCAAC18550.053.2032432
CGCAGAG19250.050.866168
ACGCAGA23500.042.9094357
GCAGAGT23500.041.6669589
TACACCG1301.7277802E-533.248295
GAATACG2303.7265636E-828.1731559
GTACATG17850.025.0255531
TATAGAC2051.3547653E-524.5983283
TACATGG18400.024.2736632
CTAGAGA3952.5465852E-1123.7086984
TTACACC3803.929017E-1022.748834
CTACACC3554.432877E-922.321624
TACACCC2954.1154453E-721.9776845
GTCTATG3004.830781E-721.614811
Produced by FastQC (version 0.11.5)