FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792654
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTTGACAGTAATAAGTTCCAAAATCATCCGGCTGCAGGCT22320.28158566032594295No Hit
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCCAAC21870.27590853007743604No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18090.228220635989978No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17190.21686637549296414No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16850.21257698819409226No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15790.19920419249760932No Hit
ATCTATTTCTGTGCGGGACATAACCATAATGACCACGGTGCCCCCAACTG15330.19340090379913558No Hit
ACTACAACCCGTCCCTTGAGAGTCGAGTCACCATATCCATGGACACGTCC15300.19302242844923512No Hit
TTCTAAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC14890.18784993200059547No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14710.1855790799011927No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14480.18267743555195584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14160.1786403651530176No Hit
GAAATAGATAGCCGAGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGA13230.16690762930610328No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12700.16022123145786182No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12520.15795037935845904No Hit
TCCATGGACACGTCCAAGAATCAGTTCTCCCTGAAACTGACCTCTGTGAC12510.1578242209084922No Hit
GTATTCCCTGGCCCCAGGGGTCGAACCAGTTGGGGGCACCGTGGTCATTA12420.15668879485879084No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12020.15164245686011804No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCCTGACTACTGGGGCTGGGTCCG11450.1444514252120093No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11450.1444514252120093No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10270.12956472811592448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9670.12199522111791526No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9670.12199522111791526No Hit
GAGTATCACTGAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCC9590.12098595351818069No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9580.12085979506821387No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9490.1197243690185125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9460.11934589366861202No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9280.11707504156920927No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9250.1166965662193088No Hit
GTAGGAGACAGAGTCACCATTACTTGCTGGGCCAGTCAGAGTATCACTGA9150.1154349817196406No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8930.11265949582037055No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8880.11202870357053644No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGCTG8880.11202870357053644No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8700.10975785147113368No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8320.10496383037239451No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8270.10433303812256041No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8270.10433303812256041No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8230.10382840432269314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7960.10042212617358898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7930.10004365082368852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13800.087.22471
TCAACGC16500.071.645594
CAACGCA17000.070.386395
ATCAACG16950.069.743493
TATCAAC17600.068.805982
AACGCAG17750.067.006226
CGCAGAG20750.057.25718
GCAGAGT23500.050.856799
ACGCAGA27100.043.8629077
CAGCGCT4500.041.597729
GTACACG9650.040.3362925
TACACGG10200.038.8679856
TATGTAC11000.036.6965182
ACACGGG10900.036.351227
GTATGTA12000.036.0389481
ACGGGGT11050.035.835149
GAATACG1501.1970096E-633.598169
CACGGGG12050.032.8654178
GTACATG20450.032.77871
GGCAGCG5950.031.480327
Produced by FastQC (version 0.11.5)