FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792654
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCCAAC22800.28764126592435035No Hit
CTACTATACTGTTGACAGTAATAAGTTCCAAAATCATCCGGCTGCAGGCT21370.26960060757909504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18390.23200538948898258No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17570.2216603965917033No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16350.20626906569575126No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16300.20563827344591712No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15280.19277011154930146No Hit
ATCTATTTCTGTGCGGGACATAACCATAATGACCACGGTGCCCCCAACTG14450.18229896020205538No Hit
TTCTAAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTC14160.1786403651530176No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13430.1694307983054397No Hit
ACTACAACCCGTCCCTTGAGAGTCGAGTCACCATATCCATGGACACGTCC13310.16791689690583786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12880.16249208355726458No Hit
TCCATGGACACGTCCAAGAATCAGTTCTCCCTGAAACTGACCTCTGTGAC12870.16236592510729778No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12310.15530105190915583No Hit
GAAATAGATAGCCGAGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGA12050.1520209322100185No Hit
GTATTCCCTGGCCCCAGGGGTCGAACCAGTTGGGGGCACCGTGGTCATTA11650.14697459421134568No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11630.14672227731141205No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCCTGACTACTGGGGCTGGGTCCG11520.145334534361777No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11480.14482990056190972No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11170.1409189886129383No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10220.12893393586609037No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10110.12754619291645536No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9950.12552765771698624No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9300.1173273584691429No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9210.11619193241944152No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8810.1111455944207687No Hit
GAGTATCACTGAGTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCC8780.11076711907086825No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8700.10975785147113368No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8680.10950553457120005No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8640.10900090077133276No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8560.1079916331715982No Hit
GTAGGAGACAGAGTCACCATTACTTGCTGGGCCAGTCAGAGTATCACTGA8450.10660389022196318No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8420.1062254148720627No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8360.1054684641722618No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8110.10231450292309129No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7980.10067444307352263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.072.89691
ATCAACG9750.071.752393
TCAACGC9800.071.381794
CAACGCA10700.065.3942645
AACGCAG11250.062.0989386
TATCAAC11500.061.4606972
CGCAGAG13250.052.7255138
GCAGAGT16250.042.991579
GGCAGCG4250.033.8926937
GTATTAG3000.033.6783641
ACGCAGA21250.033.2148367
GTACACG7300.032.6093985
TACACGG7850.031.1942256
CAGCGCT4650.030.9771889
GTATGTA9000.030.47091
GCAGCGC4850.029.6997788
ACACGGG8250.029.681787
GTACATG18700.029.3302781
TACGATG1000.00623069228.84892
TACATGG18900.028.619942
Produced by FastQC (version 0.11.5)