FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528966
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCCACTTTCGGCCCTGGGACCAAAGTGGAAATCAGAC61510.40229802363165695No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGC55800.36495252347010987No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37830.24742211403000458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29180.19084793252433344No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25150.1644902502737144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20830.13623586135989946No Hit
CCCTTATAGACAACATCGTCACTACCAACCCCAATTGGACCTCTCGCACA20670.13518940251123962No Hit
TTATTACTGTCAGCAGTATAATAAGTGGCCCACTTTCGGCCCTGGGACCA19980.13067654872639417No Hit
TTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGCT19040.12452860299051777No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18270.11949251978134241No Hit
GTCTGAAGATATTGCAGTTTATTACTGTCAGCAGTATAATAAGTGGCCCA18080.11824984989855888No Hit
GAGTATCAGCAGTTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC17800.11641854691340423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17770.11622233587928052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17540.11471805128433203No Hit
CTCCTGCAGGGCCACTCAGAGTATCAGCAGTTATTTAGCCTGGTACCAGC17530.11465264760629078No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17020.11131706002618763No Hit
GTCTCCAGCCACTGTGTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCT16770.10968196807515668No Hit
ATAATAAGTGGCCCACTTTCGGCCCTGGGACCAAAGTGGAAATCAGACGA16670.10902793129474429No Hit
TCTATGATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGC16550.10824308715824943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16290.10654259152917724No Hit
CTATAAGGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT16160.10569234371464113No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15810.10340321498319781No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15730.10287998555886789No Hit
GTAATAAACTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTAA15700.10268377452474417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27800.076.210521
ATCAACG30750.067.4799043
TCAACGC31600.065.8927844
TATCAAC32850.063.1661262
CAACGCA32950.062.7557565
AACGCAG34000.060.8177156
GGTATCA10400.054.0472561
CGCAGAG39000.052.9824268
GCAGAGT44200.047.0749749
ACGCAGA61200.034.0141687
GTACATG39900.033.593281
ACATGGG37500.033.238543
TACATGG39600.032.3855972
GTACACG9350.030.0524655
CTTATTA29650.029.8946061
ATACTGC31700.028.8574477
TATACTG32100.028.280866
GTATAAT42250.027.9723871
ATTATAC32350.027.6168734
TTATACT32150.027.5645715
Produced by FastQC (version 0.11.5)