FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528966
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCCACTTTCGGCCCTGGGACCAAAGTGGAAATCAGAC57360.37515549724454306No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGC53500.3499096775206251No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37760.24696428828371592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26620.1741045909457764No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22430.14670044984649758No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20330.13296567745783752No Hit
CCCTTATAGACAACATCGTCACTACCAACCCCAATTGGACCTCTCGCACA19790.12943387884361066No Hit
TTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGCT18210.11910009771309499No Hit
TTATTACTGTCAGCAGTATAATAAGTGGCCCACTTTCGGCCCTGGGACCA18150.11870767564484758No Hit
CTCCTGCAGGGCCACTCAGAGTATCAGCAGTTATTTAGCCTGGTACCAGC17380.11367159243567222No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17270.11295215197721858No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17150.11216730784072373No Hit
GAGTATCAGCAGTTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC16990.11112084899206393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16940.11079383060185771No Hit
GTCTGAAGATATTGCAGTTTATTACTGTCAGCAGTATAATAAGTGGCCCA16700.109224142328868No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16610.10863550922649687No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16200.1059539584268061No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15880.10386104072948646No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15730.10287998555886789No Hit
GTCTCCAGCCACTGTGTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCT15450.10104868257371322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15420.10085247153958951No Hit
GTAATAAACTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTAA15330.10026383843721835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG17500.065.082343
TCAACGC17700.063.939684
GTATCAA17950.063.8879361
CAACGCA20400.055.4770745
TATCAAC21200.055.0837172
AACGCAG21150.053.464236
CGCAGAG23850.047.7199138
GGTATCA7600.047.4506951
GTACATG38700.039.696741
GCAGAGT29300.039.0895279
TACATGG39600.037.6807252
ACATGGG38700.037.253163
CATGGGA19450.031.8734
CTTATTA28550.030.0626321
TATACTG30150.029.8604776
CATGGGG24300.029.3679074
ATACTGC31000.028.1160687
GAACCGT1804.9496066E-628.0091296
CAGCGCT7350.026.4610679
TTATACT32650.026.272895
Produced by FastQC (version 0.11.5)