FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859960
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21780.25326759384157405No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17900.20814921624261593No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17190.19989301827992No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16430.1910553979254849No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15860.18442718265965857No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13620.15837945950974464No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12160.14140192567096144No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTACTG12000.13954137401739614No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11450.13314572770826552No Hit
GCCTACATGGAGCTGAGCAGCCTAATATCTGAGGACACGGCCTTTTATTA11050.1284943485743523No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA11000.12791292618261316No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10360.12047071956835201No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10200.11861016791478672No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10040.11674961626122146No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9830.11430764221591702No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT9710.11291222847574305No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9600.11163309921391693No Hit
GATTATTACTGCTGCGCATATGCAGGCAGTAACACTTGGGTGTTCGGCGG9520.11070282338713427No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9440.10977254756035164No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9360.10884227173356902No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9070.10547002186148194No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG9010.10477231499139494No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8970.10430717707800363No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8770.10198148751104702No Hit
GCCTAATATCTGAGGACACGGCCTTTTATTACTGTGCCTTCCGGGGTATG8690.1010512116842644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.069.874991
TCAACGC11700.060.8426864
ATCAACG11900.059.8201183
TATCAAC12500.057.5239942
CAACGCA12750.055.832115
AACGCAG13450.052.9078566
GGTATCA4950.043.5940251
CGCAGAG16200.043.5488328
ACGCAGA20000.035.9944427
TACACCG1102.5565873E-432.6840865
GTACATG22950.032.5957951
ACATGGG23950.030.022963
TATTGAG9000.029.560945
GCAGAGT27350.028.690279
TTATTGA9850.028.4699974
GCAGCGC5950.027.8276358
GTTATAC1306.788245E-427.665441
CTAGTAC1306.802137E-427.6557673
TATTACG1358.480254E-426.631482
CAGAGTA17600.025.768759
Produced by FastQC (version 0.11.5)