FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812070
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGACTGCTACGTGAATATGAGCTGCAGTAATAATCAGCCTCGTCC72360.8910561897373379No Hit
GTCATAGAGTATTCGGCGGAGGGACCAAAGTGACCGTCCTCGGTCAGCCC55060.6780203677022917No Hit
GCCTTGGGCTGACCGAGGACGGTCACTTTGGTCCCTCCGCCGAATACTCT43450.535052396960853No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37920.4669548191658355No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT33660.41449628726587606No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC30780.3790313642912557No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG30590.37669166451168No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28130.34639870947085843No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA27500.3386407575701602No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGATTATAACTATGTCTCTT27140.33420764219833266No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT25870.31856859630327433No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT24590.3028064083145542No Hit
GATTATTACTGCAGCTCATATTCACGTAGCAGTCATAGAGTATTCGGCGG24490.30157498737793537No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24060.29627987735047473No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG23820.2933244671025897No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT23280.28667479404484836No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23260.2864285098575246No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21910.26980432721317127No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21320.26253894368712055No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20250.2493627396652998No Hit
GATTATAACTATGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAG20090.24739246616670976No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19750.24320563498220596No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19300.23766424076742151No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT18840.23199970445897522No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18660.22978314677306144No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18640.22953686258573766No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18310.22547317349489573No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18060.22239462115334882No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT17860.21993177928011132No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17850.21980863718644947No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17610.2168532269385644No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17040.20983412759983744No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17020.2095878434125137No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16820.20712500153927618No Hit
TTATAACTATGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAGAG16360.20146046523082986No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC16120.19850505498294485No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG15930.19616535520336917No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGATTATA14550.1791717462780302No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14240.17535434137451203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.1730146415949364No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC13870.17079808390902262No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT13840.170428657628037No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13800.1699360892533895No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG13690.16858152622310885No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13610.16759638947381383No Hit
AATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG13580.1672269631928282No Hit
GACCGAGGACGGTCACTTTGGTCCCTCCGCCGAATACTCTATGACTGCTA13180.16230127944635314No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13130.16168556897804376No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA12810.1577450219808637No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG12810.1577450219808637No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12570.1547896117329787No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT12380.15244991195340304No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12160.14974078589284173No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG12120.14924821751819423No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG12110.14912507542453238No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12110.14912507542453238No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11810.14543081261467605No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11770.14493824424002855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11710.14419939167805731No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11280.13890428165059662No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11220.13816542908862536No Hit
GTATTCGGCGGAGGGACCAAAGTGACCGTCCTCGGTCAGCCCAAGGCTGC11190.13779600280763973No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11000.13545630302806408No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGG10590.13040747718792717No Hit
CCCATAGACTACCACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT10230.12597436181609958No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT10210.12572807762877583No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGT9880.12166438853793392No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9470.11661556269779699No Hit
GTCTGGGGCAGAGGTGAAGAGGCCTGGGTCCTCGGTGAAGGTCTCCTGCA9440.11624613641681136No Hit
CTTTGGTCCCTCCGCCGAATACTCTATGACTGCTACGTGAATATGAGCTG9390.11563042594850198No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA9300.11452214710554508No Hit
GGAACCAACAGTGACGTTGGTGATTATAACTATGTCTCTTGGTACCAACA9220.11353701035625008No Hit
GCTCATATTCACGTAGCAGTCATAGAGTATTCGGCGGAGGGACCAAAGTG9160.11279815779427882No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9150.11267501570061694No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT9140.11255187360695507No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9090.1119361631386457No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9050.11144359476399816No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8940.11008903173371754No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGG8860.10910389498442254No Hit
GTTATAATCACCAACGTCACTGTTGGTTCCAGTGCAGGAGATGGTGATCG8650.10651791101752311No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGT8580.10565591636188998No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA8560.10540963217456623No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAA8540.10516334798724247No Hit
CAACAACACCCAGGCAAAGCCCCCAGAGTCATGATTTATGATGTCACTAA8400.1034393586759762No Hit
TTTATGATGTCACTAATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGC8240.10146908517738619No Hit
TCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTG8220.10122280099006244No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8200.10097651680273868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.077.286221
GTATCAA15450.077.076561
ATCAACG20750.057.5951463
TCAACGC21000.057.252324
TATCAAC21400.055.2239272
TAGTCGA400.00622047653.9954534
CAACGCA22900.052.5021255
AACGCAG23250.051.7117736
ATACGGT605.118295E-447.9959563
ACGCAGA26950.044.6121987
CTAGACC657.593263E-444.303964
CGCAGAG27350.043.9597328
ATAGTCC1656.396658E-834.906158
GCAGAGT35450.034.3215079
ATACGAG850.00283746533.8794983
TATTGAG13850.033.7877665
GTATAAC1601.9441923E-631.5751111
GTACATG25200.031.5035131
TTATTGA14900.030.923574
ACATGGG26300.030.3852733
Produced by FastQC (version 0.11.5)