FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812070
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGACTGCTACGTGAATATGAGCTGCAGTAATAATCAGCCTCGTCC61550.7579395864888495No Hit
GTCATAGAGTATTCGGCGGAGGGACCAAAGTGACCGTCCTCGGTCAGCCC47710.5875109288608125No Hit
GCCTTGGGCTGACCGAGGACGGTCACTTTGGTCCCTCCGCCGAATACTCT38140.46966394522639676No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37490.4616597091383748No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT28780.3544029455588804No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC27220.33519277894762767No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24420.3007129927223023No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGATTATAACTATGTCTCTT23470.289014493824424No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23390.288029357075129No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23220.2859359414828771No Hit
GATTATTACTGCAGCTCATATTCACGTAGCAGTCATAGAGTATTCGGCGG21590.2658637802159912No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21470.26438607509204876No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT21190.26093809646951616No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT21070.25946039134557364No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG20920.2576132599406455No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20280.24973216594628544No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19420.23914194589136403No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18930.23310798330193208No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17490.21537552181462188No Hit
GATTATAACTATGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAG17070.2102035538808231No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16710.2057704385089955No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16230.19985961801322547No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15790.1944413658921029No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT15570.1917322398315416No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15260.18791483492802344No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15230.1875454086470378No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15180.18692969817872843No Hit
TTATAACTATGTCTCTTGGTACCAACAACACCCAGGCAAAGCCCCCAGAG15090.18582141933577154No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14950.18409743002450527No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14890.18335857746253403No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14670.18064945140197275No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14550.1791717462780302No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14460.17806346743507334No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14440.1778171832477496No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14430.17769404115408768No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13590.16735010528649008No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC13420.16525668969423818No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA12970.15971529547945373No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGATTATA12760.15712931151255433No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12130.14937135961185613No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC11790.1451845284273523No Hit
AATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG11310.13927370793158225No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT11200.1379191449013016No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11120.1369340081520066No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10820.13323974534215033No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10620.13077690346891277No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10560.1300380509069415No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC10540.12979176671961776No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10510.12942234043863216No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10360.127575209033704No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10290.12671321437807084No Hit
GTATTCGGCGGAGGGACCAAAGTGACCGTCCTCGGTCAGCCCAAGGCTGC10260.12634378809708524No Hit
GACCGAGGACGGTCACTTTGGTCCCTCCGCCGAATACTCTATGACTGCTA10070.12400408831750957No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG10050.12375780413018582No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT9880.12166438853793392No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9550.11760069944709199No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9490.11686184688512073No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9140.11255187360695507No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9110.11218244732596945No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9080.1118130210449838No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9030.11119731057667441No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8870.1092270370780844No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8820.10861132660977503No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGG8600.10590220054921373No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT8130.10011452214710555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9500.066.759381
TCAACGC10800.057.3217624
ATCAACG11150.055.5224273
TATCAAC12200.051.9239432
GGTATCA4900.048.5367931
CAACGCA14100.043.906035
ACGCAGA14350.043.141127
CGCAGAG14700.043.093348
AACGCAG14900.042.0317846
GTACATG22300.035.5501171
TATTGAG14000.034.964375
ATAGGAC1708.324787E-833.8755153
ACATGGG22800.033.7826583
TTATTGA15250.032.5704774
AGGACCG1402.8829489E-530.8509185
GGTTTAT11300.029.975931
ATTGAGT10650.029.0645566
TACGAGT1255.372318E-428.7941884
TACATGG28650.028.3921742
GTGCTAG2054.2532884E-728.1247921
Produced by FastQC (version 0.11.5)