FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038302
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32510.3131073618272911No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29920.2881627888610443No Hit
TCTATAAGGATAGCAACCGGCCCTCTTGGATCCCTGAGCGATTCTCTGGC24950.24029617587175986No Hit
TGCACATACAGTGTGTTTCTTGAATCATCTCTTGAGATGCTGAATCTGCC23460.22594582308422792No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG23430.2256568898066266No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23390.2252716454364915No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23270.22411591232608624No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG22520.21689258038605336No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATGCTTGG22160.21342538105483763No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21640.2084172042430815No Hit
GTAATATACGGCTGTGTCGTCGGCTTTCAGGCTGTCCATTTGCACATACA21330.20543156037453458No Hit
ATATACGGCTGTGTCGTCGGCTTTCAGGCTGTCCATTTGCACATACAGTG20470.19714880641663024No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG20270.1952225845659548No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG18570.17884969883521365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.1701817005071742No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACTGCAGTGCTG17550.16902596739676895No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT16510.1590096137732567No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16430.15823912503298654No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT15720.15140103746308878No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15410.14841539359454187No Hit
GCCGTATATTACTGTACCGCCGAGTATAGTGGGAGCTACTCCCAGTTGGC15020.14465926098572476No Hit
GTACAGTAATATACGGCTGTGTCGTCGGCTTTCAGGCTGTCCATTTGCAC14370.13839903997102962No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14200.13676175139795552No Hit
TTACTGTACCGCCGAGTATAGTGGGAGCTACTCCCAGTTGGCTCCTAACT14110.13589495156515155No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13420.12924948618032134No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG13190.1270343310520446No Hit
GTATATTACTGTACCGCCGAGTATAGTGGGAGCTACTCCCAGTTGGCTCC12960.12481917592376784No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12880.12404868718349767No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12760.12289295407309242No Hit
CCCCTGTGCTGGTCATCTATAAGGATAGCAACCGGCCCTCTTGGATCCCT12750.12279664298055863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12570.12106304331495077No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA12450.1199073102045455No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA12260.11807739944640384No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11800.11364708918985035No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATGCTTGGATGAGCTGGG11770.11335815591224903No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11430.11008357876610081No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11280.10863891237809424No Hit
GACTATTACTGTCAAGTGTGGGACAGCAGCACTGCAGTGGTATTCGGCGG11160.10748317926768897No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11110.10700162380502011No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC11090.10680900161995258No Hit
GTATAGTGGGAGCTACTCCCAGTTGGCTCCTAACTGGCTCGACCCCTGGG11020.10613482397221617No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11010.1060385128796824No Hit
CCTTATAGATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG10890.10488277976927714No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT10730.1033418022887368No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10680.10286024682606794No Hit
GTGCTGGTCATCTATAAGGATAGCAACCGGCCCTCTTGGATCCCTGAGCG10560.10170451371566268No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10500.10112664716046006No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10470.10083771388285875No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC10440.10054878060525743No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT10430.10045246951272364No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.070.4681241
AAGCGCG451.2360595E-463.9938357
GAATACG3950.063.791329
GTATCAA21150.062.765261
ATCAACG20800.061.6094473
TCAACGC20850.061.1164174
CAACGCA21550.059.1311955
TATCAAC22300.057.4985432
AACGCAG23000.055.403366
ACGCAGA25600.049.7764557
CGCAGAG25900.049.4778638
GTTACGT450.00991015947.9953775
GTGCTAG5600.046.3795281
TACACCG855.5673918E-542.348865
GCAGAGT31000.041.8024259
CAGAGCG12800.039.3712049
GTGTAGA5950.038.8011741
CCGTTGA5400.038.662949
CCCGTTG5250.038.3963058
CTAGAGA7300.037.475844
Produced by FastQC (version 0.11.5)