FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970532
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG47470.4891131874064946No Hit
GATTATTACTGTGCGGCATGGGATGACAGCCTGAGTGGTAGAGGGGTATT38020.3917439095259095No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC31540.3249764046935083No Hit
CCCTTAGAGTCTCCTGTGCAACCTCTGGATTCACTTTCAGTAACGCCTGG26180.269748962424732No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCTCTACCACTC25420.2619182056851294No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24460.25202672348773664No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24150.24883259902816188No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23850.24574151084147663No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCC22620.23306804927606717No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGA20760.21390330251861867No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20530.21153346824215996No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20470.21091525060482294No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20460.21081221433193342No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19480.2007146595887616No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19220.19803571649363444No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18940.19515070085272818No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18900.19473855576117016No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18610.19175050384737444No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18290.1884533431149102No Hit
CCCCTCTACCACTCAGGCTGTCATCCCATGCCGCACAGTAATAATCAGCC18130.18680476274867805No Hit
GTGTATTACTGTACCACAGGAGGGCCATCACGCTTTTTGGAGTGGTTACC17970.1851561823824459No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17920.18464100101799838No Hit
GTAGAGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17610.18144687655842362No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG17550.18082865892108657No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17510.18041651382952856No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17370.17897400600907543No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16380.16877341499301415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16050.1653732179876604No Hit
TTATTACTGTGCGGCATGGGATGACAGCCTGAGTGGTAGAGGGGTATTCG16020.16506410916899183No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15990.16475500035032334No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15860.16341552880275972No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15810.1629003474383122No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15760.16238516607386463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15570.16042747688896397No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG15550.16022140434318496No Hit
GTATACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCTA15420.15888193279562135No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15210.1567181710649417No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14590.15032992214579222No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC14550.14991777705423417No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14420.14857830550667056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14330.147650979050665No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14330.147650979050665No Hit
GGCCTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC14320.1475479427777755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14320.1475479427777755No Hit
CAGTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGA13910.14332345558930568No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCTCTACCACTCA13850.1427052379519686No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13620.14033540367550992No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13550.13961414976528336No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13260.13662609785148763No Hit
TTACTGTACCACAGGAGGGCCATCACGCTTTTTGGAGTGGTTACCGCGTG13160.13559573512259257No Hit
GTACATGGGAGCTTCAGCTGTGGTAGCGAAGACAGGATTCAGGACAATCT13140.13538966257681356No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12960.1335350096648024No Hit
TTTAAAAGGTGTCCAGTGTGCGGTGCAGCTGGTGGAGTCTGGGGGAGGCT12940.13332893711902338No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12420.12797105092876895No Hit
GTACCACAGGAGGGCCATCACGCTTTTTGGAGTGGTTACCGCGTGACTAC12270.12642550683542633No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12030.12395263628607815No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11870.12230405591984603No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11830.121891910828288No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11820.12178887455539847No Hit
GTATTACTGTACCACAGGAGGGCCATCACGCTTTTTGGAGTGGTTACCGC11480.11828564127715521No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11350.1169461697295916No Hit
GCCGTGTATTACTGTACCACAGGAGGGCCATCACGCTTTTTGGAGTGGTT11250.11591580700069652No Hit
GTTATTATGTATACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTC11250.11591580700069652No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11210.11550366190913848No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC11190.11529758936335947No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGAGCG11030.11364900899712735No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10990.11323686390556932No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10950.11282471881401128No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10890.11220650117667423No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10860.11189739235800572No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTA10770.11097006590200015No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10730.1105579208104421No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10700.11024881199177358No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10690.11014577571888408No Hit
TATGTATACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCAT10630.10952755808154703No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10630.10952755808154703No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10600.10921844926287849No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10520.10839415907976244No Hit
ATGTATACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATC10510.10829112280687292No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10480.10798201398820441No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10380.10695165125930933No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10380.10695165125930933No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10290.10602432480330375No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10230.10540610716596671No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10230.10540610716596671No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10150.10458181698285066No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9900.10200591016061293No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9760.10056340234015981No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9760.10056340234015981No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG9710.1000482209757123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14400.083.631041
GGTATCA4950.077.211891
ATCAACG17350.069.787373
TCAACGC17700.068.0002064
TATCAAC18300.066.164532
CAACGCA18300.065.76395
AACGCAG19450.061.7990386
CGCAGAG22000.054.3060078
ACGCAGA27400.043.8660357
ATAACGC951.06536034E-437.932673
GGGCAGT6100.036.6475871
GTAGACG10650.036.5398185
TAGACGG10700.036.3240976
GTACATG23350.035.824871
TACATGG23400.035.728042
GGTTTAT8800.033.5981031
ACATGGG24250.033.58413
ATGGGAG12900.033.5184365
TATTGAG12400.033.1264885
TATGTAG12050.032.297862
Produced by FastQC (version 0.11.5)