FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016908
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA34840.34260719750459234No Hit
GTTTATTACTGTGCGAGATTGGATCGGTCAGATAAAGTGGGGGCTATTCC33660.331003394604035No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20940.2059183328285351No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20840.20493495970136927No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20800.20454161045050293No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20730.20385324926148676No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20400.20060811794183936No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19330.1900860254811645No Hit
GTGTTACTTGTGGGGGAAACAACATTGAAAGGAAAAGTGTGCACTGGTAC18400.18094065539852178No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18010.17710550020257487No Hit
ACCATAAAGTACGCAGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGA17190.16904184055981467No Hit
ATTCCGGACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAGCCTCCAC17080.1679601301199322No Hit
CTAGTAGTATCATACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTTC16700.16422331223670186No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16480.1620598913569369No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16360.16087984360433785No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG15910.1564546645320914No Hit
ATCATACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC15740.15478293021590941No Hit
TACTACTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC15460.15202948545984496No Hit
GTATTACTGGTAGTACCATAAAGTACGCAGACTCTGTGAAGGGGCGATTC15080.1482926675766146No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA15080.1482926675766146No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG14550.14308079000263543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.14219575418818614No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13700.13472211842172546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13510.1328537094801103No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA13250.130296939349479No Hit
GTGTATGATACTACTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCT13040.12823185578243065No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA12990.1277401692188477No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12960.12744515728069797No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12900.1268551334043984No Hit
GTGCTGGGTTTTCCTTGTTGCTTTTTTAGAAGGTGTCCAGTGTGAGGTGC12830.12616677221538233No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12780.12567508565179938No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12570.12361000208475104No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12320.12115156926683632No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12000.11800477525990552No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11370.11180952455876049No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11200.11013779024257848No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11200.11013779024257848No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11180.10994111561714531No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11150.10964610367899554No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11010.10826938130096332No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10930.10748268279923061No Hit
GTCCGGAATAGCCCCCACTTTATCTGACCGATCCAATCTCGCACAGTAAT10910.10728600817379744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10900.10718767086108086No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10790.10610596042119838No Hit
GTACGCAGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGACAACGCCA10710.10531926191946568No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA10680.1050242499813159No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC10660.10482757535588273No Hit
GTTCTGGTCATGTCTTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG10630.10453256341773297No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCA10610.1043358887922998No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10610.1043358887922998No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10490.10315584103970073No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAATATTCATAGTATGAATTGGG10470.10295916641426756No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10430.10256581716340121No Hit
GATAAAGTGGGGGCTATTCCGGACTGGGGCCAGGGAACCCTGGTCATCGT10360.10187745597438509No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC10300.10128743209808556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10240.10069740822178605No Hit
GTCTTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT10240.10069740822178605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)